Self-Assembling β-Glucan Nanomedicine for the Delivery of siRNA

Biomedicines
Kyungwoo LeeJonghoon Choi

Abstract

We aimed to design and manufacture a transporter capable of delivering small interfering RNAs (siRNAs) into the skin without causing any damage. β-glucans are unique chiral polysaccharides with well-defined immunological properties and supramolecular wrapping ability. However, the chiral properties of these polymers have hardly been applied in drug delivery systems. In this study, β-glucan nanoparticles were designed and manufactured to deliver genetic material to the target cells. The β-glucan molecules were self-assembled with an siRNA into nanoparticles of 300-400 nm in diameter via a conformational transition process, in order to construct a gene delivery system. The assembled gene nanocarriers were associated with high gene-loading ability. The expression and efficiency of siRNA were verified after its delivery via β-glucan. Our results provide evidence that β-glucan nanoparticles can be effectively used to deliver siRNA into the cells.

References

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Feb 26, 2019·Drug Delivery·Tejinder SinghJungkyun Im

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Methods Mentioned

BETA
scanning
dynamic light scattering
fluorescence microscopy
acid treatment
transfection
enzyme-linked immunosorbent assay
ELISA
protein assay
electrophoresis

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