Self-assembling chimeric protein for the construction of biodegradable hydrogels capable of interaction with integrins expressed on neural stem/progenitor cells

Biomacromolecules
Tadashi Nakaji-HirabayashiHiroo Iwata

Abstract

The poor survival of neural stem/progenitor cells following transplantation into the brain is the major problem limiting the effect of cell-based therapy for Parkinson's disease. To overcome this problem, we are involved in designing keratin-based hydrogels that serve as physical barriers to prevent the infiltration of inflammatory cells. Another feature of the hydrogels is to contain a polypeptide that promotes integrin-mediated cell adhesion. To construct such hydrogels, a chimeric protein consisting of an alpha-helical polypeptide and a globular domain derived from laminin was synthesized by means of recombinant DNA technology and coassembled with extracted keratins that form hydrogels through intermolecular coiled-coil association of alpha-helical segments. It was found that neurosphere-forming cells specifically adhered to the keratin-based composite hydrogel and actively proliferated at a high survival rate. These results suggested that the composite hydrogel provides microenvironments suitable for the survival and proliferation of neural progenitor cells.

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Citations

Jan 31, 2012·Cell and Tissue Research·Shuai WangKee Woei Ng
Sep 11, 2013·Bioscience, Biotechnology, and Biochemistry·Yuki OzakiMasayuki Hara
May 18, 2010·Angewandte Chemie·Hana Robson Marsden, Alexander Kros
May 20, 2014·Biomaterials·Raquel SilvaAldo R Boccaccini
Apr 1, 2014·Chemical Society Reviews·Jenny BrinkmannPascal Jonkheijm
Apr 27, 2018·Chemical Society Reviews·Danielle M Raymond, Bradley L Nilsson

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