Self-assembling diphenylalanine peptide nanotubes selectively eradicate bacterial biofilm infection.

Acta Biomaterialia
Simon L PorterGarry Laverty

Abstract

Biofilms present a major problem to industry and healthcare worldwide. Composed of a population of surface-attached microbial cells surrounded by a protective extracellular polysaccharide matrix, they are responsible for increased tolerance to antibiotics, treatment failure and a resulting rise in antimicrobial resistance. Here we demonstrate that self-assembled peptide nanostructures composed of a diphenylalanine motif provide sufficient antibacterial activity to eradicate mature biofilm forms of bacteria widely implicated in hospital infections. Modification of terminal functional groups to amino (-NH2), carboxylic acid (-COOH) or both modalities, and switch to d-isomers, resulted in changes in antibacterial selectivity and mammalian cell toxicity profiles. Of the three peptide nanotubes structures studied (NH2-FF-COOH, NH2-ff-COOH and NH2-FF-NH2), NH2-FF-COOH demonstrated the most potent activity against both planktonic (liquid, free-floating) and biofilm forms of bacteria, possessing minimal mammalian cell toxicity. NH2-FF-COOH resulted in greater than 3 Log10 CFU/mL viable biofilm reduction (>99.9%) at 5 mg/mL and total biofilm kill at 10 mg/mL against Staphylococcus aureus after 24 h exposure. Scanning electron microscopy...Continue Reading

Citations

Jun 18, 2020·Frontiers in Bioengineering and Biotechnology·Seema GuptaPradeep Kumar
Apr 17, 2019·Pharmaceutics·Lucia LombardiStefania Galdiero
Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrew W SimonsonScott H Medina
Oct 11, 2020·International Journal of Molecular Sciences·Sarah Ya'ariLihi Adler-Abramovich
May 1, 2021·International Journal of Molecular Sciences·Justyna SawickaSylwia Rodziewicz-Motowidło
Dec 16, 2020·ACS Biomaterials Science & Engineering·Neelam Chauhan, Yashveer Singh
Oct 14, 2019·ACS Biomaterials Science & Engineering·Monessha NambiarJean Chmielewski
Sep 30, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Harsimran KaurSangita Roy
Jan 26, 2021·ACS Medicinal Chemistry Letters·Chaitanya Kumar ThotaBeate Koksch
May 29, 2019·ACS Applied Materials & Interfaces·Lee SchnaiderLihi Adler-Abramovich
Sep 2, 2021·Chemistry : a European Journal·Souvik MisraJayanta Nanda
Oct 15, 2021·Nanotechnology·Chenlei WangYeteng Zhong

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