Self-assembly behaviours of peptide-drug conjugates: influence of multiple factors on aggregate morphology and potential self-assembly mechanism

Royal Society Open Science
Qin FanZhipeng Chen

Abstract

Peptide-drug conjugates (PDCs) as self-assembly prodrugs have the unique and specific features to build one-component nanomedicines. Supramolecular structure based on PDCs could form various morphologies ranging from nanotube, nanofibre, nanobelt to hydrogel. However, the assembly process of PDCs is too complex to predict or control. Herein, we investigated the effects of extrinsic factors on assembly morphology and the possible formation of nanostructures based on PDCs. To this end, we designed a PDC consisting of hydrophobic drug (S)-ketoprofen (Ket) and valine-glutamic acid dimeric repeats peptide (L-VEVE) to study their assembly behaviour. Our results showed that the critical assembly concentration of Ket-L-VEVE was 0.32 mM in water to form various nanostructures which experienced from micelle, nanorod, nanofibre to nanoribbon. The morphology was influenced by multiple factors including molecular design, assembly time, pH and hydrogen bond inhibitor. On the basis of experimental results, we speculated the possible assembly mechanism of Ket-L-VEVE. The π-π stacking interaction between Ket molecules could serve as an anchor, and hydrogen bonded-induced β-sheets and hydrophilic/hydrophobic balance between L-VEVE peptide play s...Continue Reading

References

Feb 6, 2009·Nano Letters·Honggang CuiSamuel I Stupp
Feb 2, 2010·Cancer Biology & Therapy·Ying HuangM Saeed Sheikh
Apr 17, 2012·Biophysical Journal·Paolo ArosioMassimo Morbidelli
Apr 25, 2013·Chemical Communications : Chem Comm·Ran LinHonggang Cui
Apr 4, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Ian W HamleyJanne Ruokolainen
Jul 3, 2016·Advanced Drug Delivery Reviews·Yin WangHonggang Cui
Jun 9, 2017·Organic & Biomolecular Chemistry·C J BuettnerJ E Goldberger
Jun 21, 2017·International Journal of Pharmaceutics·Xi-Rui ZhouShi-Zhong Luo
Jun 29, 2017·Theranostics·Zhipeng ChenHonggang Cui
Jul 1, 2017·Organic & Biomolecular Chemistry·Charlotte J C Edwards-Gayle, Ian W Hamley
Jul 29, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Meryem Hatip KocMustafa O Guler
Feb 9, 2016·Macromolecules·Myungshim KangSharon M Loverde

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Citations

Apr 17, 2019·Pharmaceutics·Lucia LombardiStefania Galdiero
Mar 12, 2021·Advanced Drug Delivery Reviews·Julie MouginPatrick Couvreur
May 1, 2021·International Journal of Molecular Sciences·Justyna SawickaSylwia Rodziewicz-Motowidło
Apr 28, 2020·ACS Omega·Chaitanya Kumar ThotaBeate Koksch

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Methods Mentioned

BETA
transmission electron microscopy
circular dichroism

Software Mentioned

VEVE
Ket

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