Self-assembly DNA-conjugated polymer for detection of single nucleotide polymorphism

Biotechnology and Bioengineering
Shu Taira, Kenji Yokoyama

Abstract

We developed a self-assembly DNA-conjugated polymer based on polyacrylic acid (PAA) for DNA chip fabrication. A 20-mer single-stranded DNA (ssDNA, probe-1), and 3-(2-pyridyldithio)propionyl hydrazide (PDPH), for promoting self-assembled immobilization, were both covalently attached to PAA as sidechains. This DNA-conjugated PAA was then spontaneously immobilized on a gold substrate. Probe-1 on the immobilized polymer was hybridized to a 34-mer ssDNA (probe-2), which had the sequence desired for analyzing the target DNA. The fluorescence intensity after incubating the P-1 DNA-conjugated polymer with probe-2 DNA was much higher than with control sequence in the first hybridization. The interactions between target DNA and the DNA-conjugated PAA were investigated by fluorescence measurement. The interaction of fully matched target DNA with this immobilized DNA conjugated polymer has been studied at different ion strength conditions. SNP sequences as targets showed less than 15% the intensity of fully matched target DNA in the second hybridization, indicating that the gold surfaces coated with the DNA-conjugated PAA was highly specific to fully matched DNA. The DNA-conjugated PAA immobilized on a gold substrate is characterized by re...Continue Reading

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Citations

Aug 23, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Cuie WangShou-Jun Xiao
Mar 23, 2015·The Journal of Surgical Research·Mitsuhiro HishidaShuji Nomoto
Sep 17, 2010·Angewandte Chemie·Minseok Kwak, Andreas Herrmann
May 18, 2013·Chemical Science·Lu PengWeihong Tan
Apr 22, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Hugh D BurrowsAndrej Jamnik
Dec 22, 2007·Chemical Reviews·Audrey SassolasLoïc J Blum
Nov 26, 2013·The Journal of Physical Chemistry. B·Jie YangKirk S Schanze

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