Self-assembly of a dual functional bioactive peptide amphiphile incorporating both matrix metalloprotease substrate and cell adhesion motifs

Soft Matter
Ashkan DehsorkhiJanne Ruokolainen

Abstract

We describe a bioactive lipopeptide that combines the capacity to promote the adhesion and subsequent self-detachment of live cells, using template-cell-environment feedback interactions. This self-assembling peptide amphiphile comprises a diene-containing hexadecyl lipid chain (C16e) linked to a matrix metalloprotease-cleavable sequence, Thr-Pro-Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln, and contiguous with a cell-attachment and signalling motif, Arg-Gly-Asp-Ser. Biophysical characterisation revealed that the PA self-assembles into 3 nm diameter spherical micelles above a critical aggregation concentration (cac). In addition, when used in solution at 5-150 nM (well below the cac), the PA is capable of forming film coatings that provide a stable surface for human corneal fibroblasts to attach and grow. Furthermore, these coatings were demonstrated to be sensitive to metalloproteases expressed endogenously by the attached cells, and consequently to elicit the controlled detachment of cells without compromising their viability. As such, this material constitutes a novel class of multi-functional coating for both fundamental and clinical applications in tissue engineering.

References

Jan 1, 1995·Methods in Enzymology·R W Woody
Apr 1, 1997·The Journal of Clinical Investigation·R C BillinghurstA R Poole
Jul 19, 2000·Biochimica Et Biophysica Acta·L C Serpell
Nov 27, 2001·Science·J D HartgerinkS I Stupp
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey D HartgerinkSamuel I Stupp
Apr 23, 2004·Chemical Society Reviews·Dennis W P M Löwik, Jan C M van Hest
Jan 27, 2005·Journal of the American Chemical Society·Heather A BehannaSamuel I Stupp
May 19, 2005·Journal of the American Chemical Society·John D TovarSamuel I Stupp
Jun 14, 2006·Biomacromolecules·Mustafa O GulerSamuel I Stupp
Oct 16, 2007·Angewandte Chemie·Ian W Hamley
Feb 22, 2008·Accounts of Chemical Research·Ziad GanimAndrei Tokmakoff
Feb 6, 2009·Nano Letters·Honggang CuiSamuel I Stupp
Feb 28, 2009·Biochemistry·Dimitris MissirlisMatthew Tirrell
May 8, 2009·Chemical Society Reviews·Elsie S PlaceMolly M Stevens
May 22, 2009·Nature Materials·Elsie S PlaceMolly M Stevens
Aug 22, 2009·Clinics in Dermatology·Lijuan Zhang, Timothy J Falla
Oct 13, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Megan A GreenfieldSamuel I Stupp
Jan 5, 2010·Soft Matter·Alvaro MataSamuel I Stupp
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ramille N ShahSamuel I Stupp
Feb 20, 2010·Bioconjugate Chemistry·Dimitris MissirlisMatthew Tirrell
Apr 10, 2010·Journal of the American Chemical Society·E Thomas PashuckSamuel I Stupp
Jun 5, 2010·Chemical Society Reviews·Dennis W P M LöwikJan C M van Hest
Oct 30, 2010·Chemical Communications : Chem Comm·Valeria CastellettoDganit Danino
Nov 3, 2011·ACS Nano·Stephen SoukaseneSamuel I Stupp
Jan 26, 2009·Advanced Materials·Erik D SpoerkeSamuel I Stupp
Nov 30, 2011·Current Opinion in Solid State & Materials Science·John B MatsonSamuel I Stupp
Jul 28, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Pasquale PalladinoIan W Hamley
Jan 17, 2013·Molecular Pharmaceutics·Roanne R JonesIan W Hamley
Feb 6, 2013·Journal of the American Chemical Society·Andrew G CheethamHonggang Cui
Mar 29, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·I W HamleyV Castelletto
May 9, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Ashkan DehsorkhiJanne Ruokolainen
Jul 5, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Valeria CastellettoEllen S Baker
Oct 24, 2013·Investigative Ophthalmology & Visual Science·Ricardo Martins Gouveia, Che John Connon
Feb 6, 2014·Advanced Materials·Job Boekhoven, Samuel I Stupp
Jan 1, 2013·Faraday Discussions·Valeria CastellettoIan W Hamley
Apr 15, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Ashkan DehsorkhiIan W Hamley
May 27, 2014·Nature Materials·Julia H OrtonySamuel I Stupp
Jun 13, 2014·Angewandte Chemie·Ian W Hamley
Sep 25, 2014·Chemical Communications : Chem Comm·Martin Conda-SheridanSamuel I Stupp
Nov 11, 2014·Chemical Communications : Chem Comm·Ian W HamleyJanne Ruokolainen

❮ Previous
Next ❯

Citations

Aug 11, 2015·Journal of Functional Biomaterials·Martina MiottoChe J Connon
Sep 29, 2015·Journal of Materials Science. Materials in Medicine·Ricardo M GouveiaChe J Connon
Dec 13, 2016·Physical Chemistry Chemical Physics : PCCP·B M SoaresW A Alves
Apr 3, 2020·The Journal of Physical Chemistry. B·Santosh L GawaliPuthusserickal Abdulrahiman Hassan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.