Self-assembly of a viral molecular machine from purified protein and RNA constituents

Molecular Cell
Minna M PoranenD H Bamford

Abstract

We present the assembly of the polymerase complex (procapsid) of a dsRNA virus from purified recombinant proteins. This molecular machine packages and replicates viral ssRNA genomic precursors in vitro. After addition of an external protein shell, these in vitro self-assembled viral core particles can penetrate the host plasma membrane and initiate a productive infection. Thus, a viral procapsid has been assembled and rendered infectious using purified components. Using this system, we have studied the mechanism of assembly of the common dsRNA virus shell and the incorporation of a symmetry mismatch within an icosahedral capsid. Our work demonstrates that this molecular machine, self-assembled under defined conditions in vitro, can function in its natural environment, the cell cytoplasm.

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Citations

Mar 11, 2004·Virus Research·Eugene V Makeyev, Jonathan M Grimes
Mar 11, 2004·Virus Research·Minna M Poranen, Roman Tuma
May 6, 2003·Journal of Molecular Biology·Denis E KainovRoman Tuma
Jul 31, 2003·Journal of Molecular Biology·José R CastónJosé L Carrascosa
Aug 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sofia Lourenco, Polly Roy
Oct 23, 2008·Nucleic Acids Research·Minna M PoranenJonathan M Grimes
Dec 21, 2011·Photochemistry and Photobiology·Alvin KatzPaul Gottlieb
Sep 6, 2012·Neuropsychobiology·Agnieszka Remlinger-MolendaJanusz K Rybakowski
Oct 4, 2013·Journal of Virology·Xiaoyu SunMinna M Poranen
Jul 4, 2012·Current Opinion in Virology·Koki Taniguchi, Satoshi Komoto
Oct 25, 2011·Journal of Molecular Biology·Daniel NemecekJ Bernard Heymann
Sep 17, 2008·Journal of Molecular Biology·Minna M PoranenDennis H Bamford
Jul 24, 2008·Journal of Molecular Biology·Ertan EryilmazRanajeet Ghose
Apr 14, 2005·Journal of Molecular Biology·Nelli J StrömstenJaana K H Bamford
Jul 8, 2014·Virology·Venkata ChelikaniKiran Kondabagil
Apr 30, 2010·The Journal of General Virology·Virginija Cvirkaitė-KrupovičDennis H Bamford
Jul 21, 2004·Virology·Simonas LaurinaviciusPentti Somerharju
Nov 3, 2016·RNA·Kalle J HanhijärviMinna M Poranen
Sep 22, 2017·The Journal of General Virology·Minna M Poranen Ictv Report Consortium
Jul 25, 2018·Viruses·Julie CallananColin Hill
Jun 21, 2003·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Dennis H Bamford
Sep 11, 2003·The Journal of Biological Chemistry·Denis E KainovEugene V Makeyev
Oct 8, 2003·The Journal of Biological Chemistry·Jíri LísalRoman Tuma
Mar 14, 2017·Nature Communications·Zhaoyang SunJuha T Huiskonen
Sep 9, 2020·Nature Communications·Geoff SuttonMark Boyce
Apr 21, 2007·Uirusu·Satoshi Komoto, Koki Taniguchi

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