Self-assembly of virus-like particles of porcine circovirus type 2 capsid protein expressed from Escherichia coli.

Virology Journal
Shuanghui YinXiangtao Liu

Abstract

Porcine circovirus 2 (PCV2) is a serious problem to the swine industry and can lead to significant negative impacts on profitability of pork production. Syndrome associated with PCV2 is known as porcine circovirus closely associated with post-weaning multisystemic wasting syndrome (PMWS). The capsid (Cap) protein of PCV2 is a major candidate antigen for development of recombinant vaccine and serological diagnostic method. The recombinant Cap protein has the ability to self-assemble into virus-like particles (VLPs) in vitro, it is particularly opportunity to develop the PV2 VLPs vaccine in Escherichia coli,(E.coli ), because where the cost of the vaccine must be weighed against the value of the vaccinated pig, when it was to extend use the VLPs vaccine of PCV2. In this report, a highly soluble Cap-tag protein expressed in E.coli was constructed with a p-SMK expression vector with a fusion tag of small ubiquitin-like modifiers (SUMO). The recombinant Cap was purified using Ni2+ affinity resins, whereas the tag was used to remove the SUMO protease. Simultaneously, the whole native Cap protein was able to self-assemble into VLPs in vitro when viewed under an electron microscope. The Cap-like particles had a size and shape that rese...Continue Reading

References

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Citations

Sep 25, 2012·Molecular Biotechnology·Andris Zeltins
Mar 22, 2014·Applied Microbiology and Biotechnology·Hu DongShi-Qi Sun
Oct 12, 2015·Applied Microbiology and Biotechnology·Dan YanShi-Qi Sun
Apr 25, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mindaugas ZaveckasAlma Gedvilaite
Dec 24, 2011·Biochimie·Sandra P Sánchez-RodríguezIsmael Bustos-Jaimes
Feb 14, 2013·Journal of Virological Methods·Edward I PattersonShane R Raidal
Jul 30, 2014·Journal of Virological Methods·Jackeline G SilvaAntonio C Freitas
Nov 22, 2011·Research in Veterinary Science·Fuxiao LiuZhiliang Wang
Sep 2, 2016·Journal of the American Chemical Society·James E NobleMaxim G Ryadnov
May 28, 2020·Bioscience Reports·Mingfang BiXiaobing Mo

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Datasets Mentioned

BETA
FJ948168
FJ948167

Methods Mentioned

BETA
PCR
electron microscopy
transmission electron microscopy

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