PMID: 9188587Jul 1, 1997Paper

Self-association of herpes simplex virus type 1 ICP35 is via coiled-coil interactions and promotes stable interaction with the major capsid protein

Journal of Virology
A PelletierM G Cordingley

Abstract

The ordered copolymerization of viral proteins to form the herpes simplex virus (HSV) capsid occurs within the nucleus of the infected cell and is a complex process involving the products of at least six viral genes. In common with capsid assembly in double-stranded DNA bacteriophages, HSV capsid assembly proceeds via the assembly of an outer capsid shell around an interior scaffold. This capsid intermediate matures through loss of the scaffold and packaging of the viral genomic DNA. The interior of the HSV capsid intermediate contains the viral protease and assembly protein which compose the scaffold. Proteolytic processing of these proteins is essential for and accompanies capsid maturation. The assembly protein (ICP35) is the primary component of the scaffold, and previous studies have demonstrated it to be capable of intermolecular association with itself and with the major capsid protein, VP5. We have defined structural elements within ICP35 which are responsible for intermolecular self-association and for interaction with VP5. Yeast (Saccharomyces cerevisiae) two-hybrid assays and far-Western studies with purified recombinant ICP35 mapped a core self-association domain between Ser165 and His219. Site-directed mutations in...Continue Reading

References

Oct 1, 1992·The Journal of General Virology·M D DavisonA J Davison
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Citations

May 29, 1999·The Journal of Biological Chemistry·B Greene, J King
Jul 5, 2002·Journal of Virology·David A McClellandFrazer J Rixon
Jan 30, 2013·Genetics and Molecular Research : GMR·M L LiM S Cai
Oct 8, 2015·MBio·Anastasia A AksyukAlasdair C Steven
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z H ZhouF J Rixon
Aug 2, 2017·The Journal of General Virology·Martin Zühlsdorf, Winfried Hinrichs
Jul 27, 2001·Journal of Molecular Biology·M BuissonR W Ruigrok
Jul 29, 1998·Journal of Molecular Biology·M H ParkerP E Prevelige
Jun 9, 2004·Virology·Zdenek KnejzlíkTomás Ruml

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