PMID: 7025897Jul 21, 1981Paper

Self-association of insulin and the role of hydrophobic bonding: a thermodynamic model of insulin dimerization

Biochemistry
Y Pocker, S B Biswas

Abstract

The association constant for insulin dimerization calculated from concentration-dependent circular dichroic (CD) spectra of insulin, K12 = 7.5 X 10(5) M-1, is used along with other association constants (K24, K46, and K26) in an attempt to decipher the complex association behavior in solution and in crystal of this protein hormone. The free-energy change associated with dimerization, -RT ln K12, is -8.01 kcal mol-1, a value which is used to test a semiquantitative thermodynamic model of the process based in part on the X-ray crystallographic data of insulin. By delineation of the hydrophobic core on the surface of insulin, which is implicated in the dimer formation, the free energy of association, delta G degrees assoc, is estimated as -8.27 kcal mol-1 by using the thermodynamic parameters of Némethy & Scheraga [Némethy, G., & Scheraga, H. A. (1962) J Phys. Chem. 66, 1773-1789] and as -10.27 kcal mol-1 by using the values of Nozaki & Tanford [Nozaki, Y., & Tanford, C. (1971) J. Biol. Chem. 246, 2211-2217]. The role of hydrophobic bonding in the dimerization of insulin is discussed, and the calculated values of free energy of association are compared with the experimental value. The importance of this thermodynamic model is deli...Continue Reading

References

Feb 5, 1976·Nature·R A PullenS Gammeltoft
Jun 1, 1979·Canadian Journal of Biochemistry·E J DodsonC D Reynolds
Oct 23, 1973·Biochemistry·R S LordJ A Rupley
Jun 25, 1971·Nature·T L BlundellM Vijayan
Nov 29, 1965·Biochimica Et Biophysica Acta·P D Jeffrey, J H Coates
Mar 28, 1953·Nature·E FREDERICQ
Nov 1, 1956·Archives of Biochemistry and Biophysics·E FREDERICQ

❮ Previous
Next ❯

Citations

Nov 28, 2007·Journal of Biomolecular NMR·Wojciech BocianLech Kozerski
May 17, 2011·The Protein Journal·Shima JaliliAli Akbar Moosavi-Movahedi
Dec 31, 2010·Pharmaceutical Research·Charlotte PinholtLene Jorgensen
Oct 11, 1991·Biochimica Et Biophysica Acta·J A WeiC L Tsou
Sep 6, 2006·Journal of the American Society for Mass Spectrometry·Raghu K ChittaMichael L Gross
Mar 1, 2012·Blood·Alexandre KauskotMarc F Hoylaerts
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·C ClementiA Maritan
Mar 20, 2015·Drug Delivery and Translational Research·Nilam H Patil, Padma V Devarajan
Nov 9, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Yisheng XuPaul L Dubin
Jun 10, 2014·Journal of Pharmaceutical Sciences·Brandon M TeskaJohn F Carpenter
Oct 1, 1995·Journal of Pharmaceutical Sciences·P KurtzhalsA R Sørensen
Jan 13, 2015·Journal of Pharmaceutical Sciences·Brandon M TeskaMichael F Wempe
Mar 1, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Lene JorgensenJens T Bukrinsky
Nov 26, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Tue SøeborgMorten Colding-Jørgensen
Apr 15, 2008·Journal of Chromatography. a·Chi-Ming YuNien-Hwa Linda Wang
Nov 25, 2014·Biophysical Journal·Tae Su ChoiHugh I Kim
Jul 9, 2009·Biotechnology Progress·Shaoling ZhangAkihiko Tanioka
Jul 15, 2009·Biotechnology Progress·Shaoling ZhangHidetoshi Matsumoto
Feb 9, 2008·Proteins·Wojciech BocianLech Kozerski
Apr 27, 2011·Biotechnology and Bioengineering·D Brett LudwigTheodore W Randolph
Jan 23, 2015·Biotechnology Journal·Mariam M Nuhu, Robin Curtis
Feb 15, 1986·Archives of Biochemistry and Biophysics·E Helmerhorst, G B Stokes
Aug 5, 2006·Journal of Chromatography. a·Chi-Ming YuNien-Hwa Linda Wang
Mar 21, 2006·Journal of Colloid and Interface Science·Chi-Ming YuNien-Hwa Linda Wang
Dec 1, 1990·Archives of Biochemistry and Biophysics·N C KaarsholmP Hougaard
May 29, 2012·International Journal of Biological Macromolecules·Reza YousefiAli-Akbar Moosavi-Movahedi
Feb 3, 2009·Biophysical Journal·Christoph JeworrekClaus Czeslik
Oct 2, 1990·Biochemistry·D N BremsB H Frank
Jan 15, 2016·European Journal of Mass Spectrometry·Uday Kumar Boga RajaLaurence A Angel
May 14, 2020·Mass Spectrometry Reviews·Alan McKenzie-CoeLisa M Jones
Oct 1, 1990·International Journal of Peptide and Protein Research·M DatheB Mehlis
Aug 1, 1996·International Journal of Peptide and Protein Research·G VecchioG Carrea
Mar 21, 2020·Chemistry : a European Journal·Mads ØstergaardKnud J Jensen
Jan 1, 1982·CRC Critical Reviews in Biochemistry·T L BlundellS P Wood
Aug 14, 2008·Biomacromolecules·Katie GigerPaul L Dubin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.