PMID: 38779Jun 1, 1979

Self-association of unconjugated bilirubin-IX alpha in aqueous solution at pH 10.0 and physical-chemical interactions with bile salt monomers and micelles

The Biochemical Journal
M C Carey, A P Koretsky


Spectrophotometric measurements of bilirubin-IX alpha in water and in aqueous/organic solvent mixtures at pH 10.0 as a function of bilirubin-IX alpha concentration (approx. 0.6--400 microM) are consistent with the formation of dimers (KD - 1.5 microM) in dilute (less than 10 microM) aqueous solution and further self-aggregation to multimers at higher concentrations. Added urea (to 10M) and increases in temperature (to 62 degrees C) obliterate the dimer-multimer transition at 10 microM, but added NaCl (to 0.30 M) promotes strong aggregation of dimers over a narrow concentration range, suggesting a 'micellization' phenomenon. Concentrations of dioxan or ethanol greater than 60% (v/v) in water were required to obtain the absorption spectrum of bilirubin-IX alpha monomers, suggesting that both hydrophobic and electrostatic (pi-orbital) interactions are involved in stabilizing the dimeric state in water. Micellar concentrations of sodium dodecyl sulphate induced spectrophotometric shifts in the dimer absorption spectrum of bilirubin-IX alpha consistent with progressive partitioning of bilirubin-IX alpha monomers into a relatively non-polar region of the micelles and allowed a deduction of the apparent critical micellar concentration...Continue Reading


Nov 2, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Marvin D Berman, Martin C Carey
Sep 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A Reuben
Sep 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J D Ostrow, L Celic
Mar 1, 1986·Clinical and Experimental Pharmacology & Physiology·S P LeeY S Choong
Mar 8, 2012·Clinical Chemistry·Andrew H HuberAlan M Kleinfeld
Sep 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J D Ostrow

Related Concepts

Macromolecular Compounds
Bile Acid Measurement
Ethanol Measurement
Indirect reacting bilirubin
Bilirubin, (4E,15E)-Isomer
Serum Bilirubin Measurement

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