Self-calibrating viscosity probes: design and subcellular localization

Bioorganic & Medicinal Chemistry
Marianna DakanaliEmmanuel A Theodorakis

Abstract

We describe the design, synthesis and fluorescence profiles of new self-calibrating viscosity dyes in which a coumarin (reference fluorophore) has been covalently linked with a molecular rotor (viscosity sensor). Characterization of their fluorescence properties was made with separate excitation of the units and through resonance energy transfer from the reference to the sensor dye. We have modified the linker and the substitution of the rotor in order to change the hydrophilicity of these probes thereby altering their subcellular localization. For instance, hydrophilic dye 12 shows a homogeneous distribution inside the cell and represents a suitable probe for viscosity measurements in the cytoplasm.

References

Dec 15, 1978·Biochimica Et Biophysica Acta·M Shinitzky, Y Barenholz
Jul 1, 1985·The Journal of Cell Biology·P A Valberg, D F Albertini
Jul 27, 1993·Biochemistry·T IwakiM Nakanishi
Mar 3, 1998·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·W MöllerJ Heyder
Apr 4, 2007·Pathophysiology : the Official Journal of the International Society for Pathophysiology·O G LunevaE I Chazov
Aug 10, 2010·Tetrahedron·Jeyanthy SutharsanEmmanuel A Theodorakis

❮ Previous
Next ❯

Citations

Apr 30, 2016·Journal of Materials Chemistry. C, Materials for Optical and Electronic Devices·Mark A Haidekker, Emmanuel A Theodorakis
Nov 4, 2016·Chemical Communications : Chem Comm·Michael R DentMarina K Kuimova
Nov 8, 2016·Chemical Communications : Chem Comm·Seung-Chul LeeO-Pil Kwon
Feb 20, 2015·Chemistry : a European Journal·Marta Dal MolinStefan Matile
Jan 31, 2020·SLAS Technology·Charles McRae WhiteWilliam S Kisaalita
Jul 1, 2017·ChemPlusChem·Mariano MacchioneStefan Matile
Jul 25, 2019·Chemistry : a European Journal·Olena VakuliukDaniel T Gryko
Jul 1, 2017·Critical Reviews in Food Science and Nutrition·Fatemah M AlhassawiRichard D Ludescher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

© 2021 Meta ULC. All rights reserved