Self contained high pressure cell, apparatus and procedure for the preparation of encapsulated proteins dissolved in low viscosity fluids for NMR spectroscopy

The Review of Scientific Instruments
Ronald W Peterson, A Joshua Wand

Abstract

The design of a sample cell for high performance nuclear magnetic resonance (NMR) at elevated pressure is described. The cell has been optimized for the study of encapsulated proteins dissolved in low viscosity fluids but is suitable for more general NMR spectroscopy of biomolecules at elevated pressure. The NMR cell is comprised of an alumina toughened zirconia tube mounted on a self-sealing non-magnetic metallic valve. The cell has several advantages including relatively low cost, excellent NMR performance, high pressure tolerance, chemical inertness and a relatively large active volume. Also described is a low volume sample preparation device which allows for the preparation of samples under high hydrostatic pressure and their subsequent transfer to the NMR cell.

References

Apr 28, 1981·Biochemistry·A A Paladini, G Weber
Jan 1, 1995·Methods in Enzymology·C R Robinson, S G Sligar
Jul 9, 1998·Journal of Magnetic Resonance·L BallardJ Jonas
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·A J WandP F Flynn
Jun 26, 1999·Journal of Biomolecular NMR·M R EhrhardtA J Wand
Apr 15, 2000·Journal of Molecular Biology·M W LassalleK Akasaka
Feb 20, 2002·Biochemistry·James K KranzA Joshua Wand
May 2, 2002·Biochimica Et Biophysica Acta·Jiri Jonas
Aug 23, 2003·Journal of Magnetic Resonance·Wen-Jin WuJohn L Markley
Jul 31, 2004·Methods : a Companion to Methods in Enzymology·Yuji O KamatariKazuyuki Akasaka
Jul 21, 2005·Journal of the American Chemical Society·Ronald W PetersonA Joshua Wand
Jul 21, 2006·The Journal of Physical Chemistry. B·Matthias MeierEike Brunner

❮ Previous
Next ❯

Citations

Jul 13, 2011·Journal of Biomolecular NMR·Nathaniel V NucciA Joshua Wand
Aug 3, 2011·The Review of Scientific Instruments·J DiefenbacherR F Marzke
Apr 25, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Ryo KitaharaKazuyuki Akasaka
Apr 3, 2010·Journal of Magnetic Resonance·Markus Beck ErlachHans Robert Kalbitzer
Nov 2, 2015·The Review of Scientific Instruments·N J BeachC R Landis
Sep 11, 2012·Current Opinion in Structural Biology·Roger FourmeKazuyuki Akasaka
Mar 25, 2014·Journal of Magnetic Resonance·Nathaniel V NucciA Joshua Wand
Jun 18, 2016·The Journal of Physical Chemistry. B·Paul Hendrik SchummelRoland Winter
Oct 27, 2016·Journal of the American Chemical Society·Yi ZhangCatherine A Royer
Apr 2, 2017·Journal of Magnetic Resonance·Luan M Nguyen, Julien Roche
Jan 20, 2017·Journal of Computational Chemistry·Qi HuangToshiko Ichiye
Sep 16, 2017·Journal of Biomolecular NMR·Markus Beck ErlachHans Robert Kalbitzer
Apr 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Cyril CharlierAdriaan Bax
Oct 5, 2018·Journal of the Royal Society, Interface·Julien Roche, Catherine A Royer
Feb 6, 2020·Journal of Biomolecular NMR·Matthias DreydoppelUlrich Weininger
Mar 12, 2020·Nature Catalysis·John B StillerDorothee Kern
Nov 16, 2016·Physical Biology·Toshiko Ichiye
Aug 31, 2017·Scientific Reports·Mimi GaoRoland Winter
Jun 24, 2016·Journal of Biomolecular NMR·Markus Beck ErlachHans Robert Kalbitzer
Jun 24, 2020·Journal of Biomolecular NMR·Markus Beck ErlachHans Robert Kalbitzer
Dec 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cécile DuboisChristian Roumestand
Apr 13, 2021·Journal of Applied Crystallography·Durgesh K RaiSol M Gruner
Feb 28, 2021·Annual Review of Biophysics·Nozomi AndoMaxwell B Watkins
Jun 21, 2021·Journal of Magnetic Resonance·Anton DuchownyAlina Adams
Sep 7, 2018·The Journal of Physical Chemistry. B·Matthias DreydoppelUlrich Weininger
Jun 21, 2018·Journal of the American Chemical Society·Cyril CharlierAd Bax
Mar 29, 2012·Journal of the American Chemical Society·Yinan FuA Joshua Wand
Jun 3, 2014·Chemical Reviews·Jerson L SilvaDebora Foguel
Jul 22, 2011·Journal of the American Chemical Society·Werner KremerHans Robert Kalbitzer
May 7, 2014·The Journal of Physical Chemistry. B·Markus Beck ErlachHans Robert Kalbitzer
Jan 25, 2014·Journal of the American Chemical Society·Kathleen G ValentineA Joshua Wand
Sep 27, 2018·The Journal of Physical Chemistry. B·Cyril CharlierAd Bax
Aug 28, 2021·Microorganisms·Ryan W PenhallurickToshiko Ichiye

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.