Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis.

Proceedings of the National Academy of Sciences of the United States of America
Takako SaitoHitoshi Sawada

Abstract

Many hermaphroditic organisms possess a self-incompatibility system to avoid self-fertilization. Recently, we identified the genes responsible for self-sterility in a hermaphroditic primitive chordate (ascidian), Ciona intestinalis: sperm-side polycystin 1-like receptors s-Themis-A/B and egg-side fibrinogen-like ligands on the vitelline coat (VC) v-Themis-A/B. Here, we investigated the sperm behavior and intracellular Ca(2+) concentration ([Ca(2+)](i)) in response to self/nonself-recognition. We found that sperm motility markedly decreased within 5 min after attachment to the VC of self-eggs but not after attachment to the VC of nonself-eggs and that the apparent decrease in sperm motility was suppressed in low Ca(2+) seawater. High-speed video analysis revealed that sperm detached from the self-VC or stopped motility within 5 min after binding to the self-VC. Because s-Themis-B contains a cation channel domain in its C terminus, we monitored sperm [Ca(2+)](i) by real-time [Ca(2+)](i) imaging using Fluo-8H-AM (AAT Bioquest, Inc.). Interestingly, we found that sperm [Ca(2+)](i) rapidly and dramatically increased and was maintained at a high level in the head and flagellar regions when sperm interacted with the self-VC but not wh...Continue Reading

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Citations

Jan 1, 2015·Nature Plants·Megumi IwanoSeiji Takayama
Sep 23, 2014·Molecular Biology and Evolution·Yutaka SatouNori Satoh
Feb 15, 2020·Scientific Reports·Hitoshi SawadaMaki Shirae-Kurabayashi

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