PMID: 16529322Mar 15, 2006Paper

Self-induction of rabbit marrow stromal stem cells into chondrocytes by transfection with reconstructed PGL3-transforming growth factor beta1 gene in vitro

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Lei LeiLi Jiang

Abstract

To explore an experimental method of transfecting the marrow stromal stem cells (MSCs) with the reconstructed PGL3-transforming growth factor-beta1 (TGF-beta1) gene and to evaluate the feasibility of self-induction of MSCs to the chondrocytes in vitro so as to provide a scientific and experimental basis for a further "gene enhanced tissue engineering" research. The rabbit MSCs was transfected with the reconstructed PGL3-TGF-beta1, gene by the Liposomes Method, the growth of the cells were observed, and the growth curve was drawn. The living activity of the transfected cells in the experimental group was evaluated by MTT, and the result was significantly different when compared with that in the control group. By the immunohistochemistry method (SABC), the antigens of TGF-beta1 and collagen II were examined at 2 and 7 days of the cell culture after transfection with PGL3-TGF-beta1 gene. The pictures of the immunohistochemistry slice were analyzed with the analysis instrument, and the statistical analysis was performed with the software of the SPSS 11.0, compared with the control group and the blank group. Transfection of the cultured rabbit MSCs in vitro with the reconstructed PGL3-TGF-beta1 gene by the Liposomes Method achieved ...Continue Reading

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Related Papers

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Jin-fu YangDong-xu Hu
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Xin-Guo LiYu Zhang
© 2021 Meta ULC. All rights reserved