Self-interaction of the major 27-kilodalton outer dense fiber protein is in part mediated by a leucine zipper domain in the rat

Biology of Reproduction
X Shao, Frans A van der Hoorn

Abstract

The RT7 gene is exclusively expressed in spermatids and encodes the 27-kDa major outer dense fiber (ODF) protein ODF27. Analysis of its amino acid structure had indicated the presence of a putative leucine zipper dimerization motif in the N-terminus and the presence of PCX repeats in the C-terminus. We had previously shown that the ODF27 N-terminal fragment can interact with full-length ODF27. We have used two different methods to analyze this interaction further. First we used fusion proteins between glutathione S-transferase (GST) and ODF27-derived fragments to show that the N-terminal half of ODF27 as well as the first 100 amino acids can interact with ODF27. A fusion protein consisting of GST and the ODF27 leucine zipper did not interact with ODF27. We found that the ODF27 C-terminal half can also interact with ODF27. The yeast two-hybrid method was next employed to analyze these interactions in vivo. We found that 1) N-terminal fragments containing the leucine zipper interact with the ODF27 N-terminus, but not with its C-terminus, 2) deletion of the leucine zipper abolished this interaction, and 3) the PCX repeats are involved in the self-interaction of the ODF27 C-terminus. The detected self-associations are weak. To anal...Continue Reading

Citations

Jul 27, 2001·Molecular Reproduction and Development·X ShaoF A van der Hoorn
Dec 16, 2004·Journal of Molecular Evolution·Erik FranckWilfried W de Jong
Feb 21, 2003·The Journal of Biological Chemistry·Bhupinder BhullarFrans A van der Hoorn
Jan 21, 2003·Biology of Reproduction·Heather A ZarskyFrans A van der Hoorn
Jun 25, 2003·Cell Stress & Chaperones·Guido KappéWilfried W de Jong
Aug 15, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Kefei YangSigrid Hoyer-Fender
Jan 5, 2002·Molecular Reproduction and Development·Christoph PetersenSigrid Hoyer-Fender
Jul 28, 2015·Biochemistry·Tatyana I Igumenova
Sep 1, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jesusa L RosalesKi-Young Lee
Apr 25, 2001·Biology of Reproduction·C J FlickingerJ C Herr
Mar 1, 2002·Biology of Reproduction·Carlos Egydio de CarvalhoYoshitake Nishimune
Apr 9, 2009·Bioscience, Biotechnology, and Biochemistry·Yoshiaki YamanoKenji Ohyama
Jul 2, 2016·Reproduction, Fertility, and Development·María Eugenia CabrillanaMiguel Walter Fornés

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