Feb 10, 2017

Self-Oligomerizing Structure of the Flagellar Cap Protein FliD and Its Implication in Filament Assembly

Journal of Molecular Biology
Wan Seok SongSung-Il Yoon

Abstract

FliD is a self-oligomerizing structural protein that caps the growing end of the bacterial flagellar filament. FliD also plays a key role in the flagellar system by continuously adding a new flagellin protein to the tip of the filament. To structurally characterize FliD oligomerization and to provide a FliD-mediated flagellin polymerization mechanism, we have determined the crystal structures of FliD proteins from Escherichia coli and Salmonella enterica serovar Typhimurium (ecFliD and stFliD, respectively). ecFliD consists of three domains (D1, D2, and D3) and forms a hexamer plate of the D2 and D3 domains that resembles a six-pointed star with legs consisting of the D1 domain. In contrast, the D2 and D3 domains of stFliD assemble into a pentamer as a five-pointed star plate. Despite their distinct oligomeric states, ecFliD and stFliD engage a common molecular surface for oligomerization. FliD also features interdomain and intersubunit flexibility, suggesting that FliD reorganizes its domains and adjacent subunits depending on the FliD binding partner. The similarity of the FliD shape to flagellin and the structural dynamics of FliD led us to propose a FliD-catalyzed filament elongation mechanism. In this model, FliD occupies ...Continue Reading

Mentioned in this Paper

Pathologic Cytolysis
Positioning Attribute
Bacterial Proteins
Molecular Helix
Alkalescens-Dispar Group
Carboxy-Terminal Amino Acid
Histidine
Protein Methylation
Pathogenic Organism
Bacterial-type Flagellum Filament

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