Self-renewal and multipotency coexist in a long-term cultured adult rat dental pulp stem cell line: an exception to the rule?

Stem Cells and Development
Andrea E SprioGiovanni Nicolao Berta

Abstract

The stemness state is characterized by self-renewal and differentiation properties. However, stem cells are not able to preserve these characteristics in long-term culture because of the intrinsic fragility of their phenotype easily undergoing senescence or neoplastic transformation. Furthermore, although isolated from the same original tissue using similar protocols, adult stem cells can display dissimilar phenotypes and important cell clone/species contamination. Finally, the lack of a clear standardization contributes to complicate the comprehension about the stemness condition. In this context, cell lines displaying a particularly stable phenotype must be identified to define one or multiple benchmarks against which other stem cell lines could be reliably assessed. The present paper demonstrates that it is possible to isolate from the rat dental pulp a stem cell line (MUR-1) that does not display neoplastic transformation in long-term culture. MUR-1 cells stably express a broad range of stemness markers and are able to differentiate into adipogenic, osteogenic, chondrogenic, neurogenic, and cardiomyogenic lineages independently of the culture passages. Moreover, serial in vitro passages have not changed their immunophenotyp...Continue Reading

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Citations

Mar 19, 2014·Biochimica Et Biophysica Acta·F Di ScipioG N Berta
Feb 4, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lucía Fernández del RíoJosé M Villalba
Aug 8, 2014·Journal of Biomaterials Applications·Ga-Hyun KimEun-Cheol Kim
Jul 3, 2021·International Journal of Molecular Sciences·Sarah Hani ShoushrahEdda Tobiasch

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Methods Mentioned

BETA
light microscopy
electrophoresis
FACS

Software Mentioned

Kodak ImageStation
GraphPad Prism

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