Self-responsive co-delivery system for remodeling tumor intracellular microenvironment to promote PTEN-mediated anti-tumor therapy.

Nanoscale
Yifei WangZhenzhong Zhang

Abstract

Delivering the pten gene into tumor cells to reacquire PTEN functionality is considered to be an attractive method for cancer treatment. However, the inhibition effect of the tumor intracellular microenvironment (TIME), especially at the high reactive oxygen species (ROS) level, on pten expression and PTEN protein functionality was nearly overlooked. Herein, the development of a potential strategy is described, which enhances PTEN-mediated anti-tumor capability by exhausting the intracellular ROS in TIME. To achieve this, poly(ethyleneimine) (PEI)-modified DSPE was introduced to protect the pten plasmid, and form liposomes for encapsulating the "scavenger" of oxidation homeostasis, epigallocatechin-3-gallate (EGCG). Notably, this was a simple system with improved safety compared which when compared with the use of PEI could accomplish efficient pten transfection and simultaneous disintegration to cause transient release of EGCG responding to the endosome environment through the "proton sponge effect". In the cytoplasm, EGCG depleted ROS and promoted the expression of the pten gene as well as restoring protein functionality, thus negatively regulating the PI3K-AKT signaling pathway. In vitro and in vivo results revealed that thi...Continue Reading

References

Jun 1, 2002·Science·Lewis C Cantley
May 3, 2003·Nature Reviews. Genetics·Clare E ThomasMark A Kay
Aug 29, 2003·The Journal of Biological Chemistry·N D SonawaneA S Verkman
Dec 24, 2003·PLoS Biology·Lloyd C TrotmanPier Paolo Pandolfi
Jun 19, 2010·Archives of Biochemistry and Biophysics·Joshua D Lambert, Ryan J Elias
Aug 11, 2011·Biochemical Pharmacology·Brahma N SinghRakesh K Srivastava
Apr 5, 2012·Nature Reviews. Molecular Cell Biology·Min Sup SongPier Paolo Pandolfi
Nov 30, 2013·Nature Reviews. Drug Discovery·Chiara GorriniTak W Mak
May 23, 2014·Current Biology : CB·Michael Schieber, Navdeep S Chandel
Oct 17, 2015·Annual Review of Medicine·Ingrid A Mayer, Carlos L Arteaga
Nov 5, 2016·Experimental & Molecular Medicine·Jongdoo KimJong-Sup Bae
Apr 12, 2017·Advanced Materials·Albert HarguindeyJennifer N Cha
Oct 22, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Binghua WangZhenzhong Zhang
Dec 5, 2017·Advanced Science·Xiaofei XinLifang Yin
Jun 15, 2018·International Journal of Pharmaceutics·Jae Min LeeEun Seong Lee
Nov 28, 2018·Nature Communications·Luke RussellBalveen Kaur
Mar 26, 2019·ACS Nano·Jonas BuckDominik Witzigmann
Apr 11, 2019·Nature Reviews. Cancer·Bryan NgoJihye Yun
Apr 25, 2019·Nature Biomedical Engineering·Mohammad Ariful IslamJinjun Shi

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Citations

Dec 4, 2020·International Journal of Nanomedicine·Xiaowei WangRongguang Shao
Oct 17, 2020·BioMed Research International·Chenglin HanXunbo Jin
Aug 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kun MaJianqiang Xu

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Methods Mentioned

BETA
transfection
electrophoresis
protein assay
flow cytometry
X-ray
fluorescence imaging

Software Mentioned

Image J

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