SEM-Raman image cytometry of cells

The Analyst
A Enciso-MartinezC Otto

Abstract

Correlative and integrated scanning electron microscopy (SEM) and Raman micro-spectroscopy is presented that enables the characterization and identification of different cancer and non-cancer cells through SEM-Raman image cytometry. The hybrid microscopy system enables the acquisition of high resolution SEM images of uncoated cells and the spatial correlation with chemical information as obtained from Raman micro-spectroscopic imaging. A sample preparation protocol and a workflow are presented that are compatible with the demands of hybrid SEM-Raman microscopy. Stainless steel cell substrates were used that are both conductive and give a low optical response in Raman scattering. Correlative and integrated SEM-Raman micro-spectroscopy is illustrated with cells from blood and cells from a SKBR-3 breast cancer cell line.

References

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Feb 4, 2016·Chemical Society Reviews·W J TippingA N Hulme

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Citations

Jun 26, 2020·Frontiers in Oncology·L G RikkertL W M M Terstappen
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Yadi WenZhongquan Sui
Jun 11, 2021·Communications Biology·Pepijn BeekmanCees Otto

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Methods Mentioned

BETA
electron microscopy
imaging
light scattering
scanning electron microscopy
flow
FACS
PCA
biopsy

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