Semaphorin 3A Inhibits Inflammation in Chondrocytes under Excessive Mechanical Stress

Mediators of Inflammation
Chikako SumiK Tanimoto

Abstract

Excessive mechanical stress causes inflammation and destruction of cartilage and is considered one of the cause of osteoarthritis (OA). Expression of semaphorin 3A (Sema3A), which is an axon guidance molecule, has been confirmed in chondrocytes. However, there are few reports about Sema3A in chondrocytes, and the effects of Sema3A on inflammation in the cartilage are poorly understood. The aim of this study was to examine the role of Sema3A in inflammation caused by high magnitude cyclic tensile strain (CTS). Expression of Sema3A and its receptors neuropilin-1 (NRP-1) and plexin-A1 (PLXA1) in ATDC5 cells was examined by Western blot analysis. ATDC5 cells were subjected to CTS of 0.5 Hz, 10% elongation with added Sema3A for 3 h. Gene expression of IL-1β, TNF-ɑ, COX-2, MMP-3, and MMP-13 was examined by qPCR analysis. Furthermore, the phosphorylation of AKT, ERK, and NF-κB was detected by Western blot analysis. Added Sema3A inhibited the gene expression of inflammatory cytokines upregulated by CTS in a dose-dependent manner. Addition of Sema3A suppressed the activation of AKT, ERK, and NF-κB in a dose-dependent manner. Sema3A reduces the gene expression of inflammatory cytokines by downregulating the activation of AKT, ERK, and NF...Continue Reading

References

Sep 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J J ParkkinenM Tammi
May 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·T AtsumiY Ikawa
Oct 1, 1994·British Journal of Rheumatology·J P Urban
Oct 6, 1997·Genes & Development·L Van Aelst, C D'Souza-Schorey
Feb 7, 1998·Science·A Hall
Feb 14, 1998·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·S B MilamJ P Schmitz
Mar 14, 1998·Osteoarthritis and Cartilage·J SteinmeyerR X Raiss
Oct 16, 1999·Cell·T TakahashiS M Strittmatter
Sep 8, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C GomezC Chenu
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Pierre Chardin
Apr 6, 2006·Genome Biology·Umar Yazdani, Jonathan R Terman
Apr 7, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lara LongobardiAnna Spagnoli
Oct 28, 2008·Cellular and Molecular Life Sciences : CMLS·L RothD Bagnard
Nov 6, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Puja GaurLee M Ellis
Dec 2, 2010·Nature Reviews. Rheumatology·Mohit KapoorHassan Fahmi
Nov 23, 2011·Biochemical Society Transactions·Carolyn A Staton
Apr 24, 2012·Nature·Mikihito HayashiHiroshi Takayanagi
Dec 23, 2014·Nature Cell Biology·Tomoyo OkadaFilippo G Giancotti
May 26, 2017·Basic Research in Cardiology·Marieke RienksAnna Papageorgiou

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Citations

Jan 19, 2019·International Journal of Molecular Sciences·Samuel Garcia
Dec 27, 2019·Cell Biology International·Naoto HiroseKotaro Tanimoto
Jun 21, 2019·Journal of Gastrointestinal Cancer·Tuba KarpuzMehmet Artac
Jun 20, 2020·Oral Diseases·Makoto YanoshitaKotaro Tanimoto
Mar 26, 2021·American Journal of Rhinology & Allergy·Honghui LiuGuolin Tan

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Methods Mentioned

BETA
protein assay
GTPases

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