Abstract
Bone regeneration is impaired in patients with type 2 diabetes mellitus (T2DM), which leads to non-healing after bone loss. The decreased osteogenic capacity of bone mesenchymal stem cells (BMSCs) might be a main reason. Sema3A, as a powerful protein promoting osteocyte differentiation, shows potential for bone regeneration treatment. BMSCs may be a therapeutic solution. In this study, we divided BMSCs from T2DM rats (BMSCs-D) and normal rats (BMSCs-N), identified their ability to differentiate into different cell types. Then we found decreased expression of Sema3A in BMSCs-D compared with BMSCs-N. Stimulating with Sema3A showed no influence in the proliferation or migration of BMSCs. However, Sema3A stimulation significantly increased the expression of osteogenic‑related genes, including type I collagen, alkaline phosphatase, Runt-related transcription factor 2 (RUNX2), bone morphogenetic protein and osteocalcin. Besides, the osteogenic capacity of BMSCs was also increased by Sema3A stimulation. In conclusion, we proved that exogenous Sema3A stimulation might repair the osteogenic capacity of BMSCs-D, thus providing a new strategy for restoring the impaired bone regeneration ability for T2DM patients.
References
Nov 1, 1992·Neuron·A L KolodkinC S Goodman
Jun 1, 1995·Neuron·Y LuoJ A Raper
Oct 22, 1993·Cell·Y LuoJ A Raper
Oct 10, 1996·Nature·O BeharM C Fishman
Jul 12, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Romain DacquinGérard Karsenty
Oct 4, 2003·Endocrinology·Hongbing HeDana T Graves
Feb 8, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·V GopalakrishnanN Srinivasan
Jul 21, 2006·Development·Stephen J Rodda, Andrew P McMahon
Feb 5, 2008·Journal of Autoimmunity·Nader G AbrahamDavid Asprinio
Jan 20, 2009·Bone·Yuki KawashimaDerek LeRoith
Feb 16, 2010·Biomaterials·Philipp NiemeyerPhilip Kasten
Apr 13, 2010·Stem Cells and Development·Christopher CramerReza Izadpanah
Sep 9, 2011·American Journal of Physiology. Endocrinology and Metabolism·Christine HamannLorenz C Hofbauer
Apr 24, 2012·Nature·Mikihito HayashiHiroshi Takayanagi
Jan 15, 2013·Diabetes Care·Ling OeiFernando Rivadeneira
Feb 5, 2013·Frontiers of Medicine·Wenbo Yan, Xin Li
Jun 26, 2014·International Orthopaedics·Nadomir GusićSaša Janković
Aug 15, 2014·Current Stem Cell Research & Therapy·Birru BhaskarParcha Sreenivasa Rao
Sep 10, 2014·Lipids·Sarawut LapmaneeNateetip Krishnamra
May 9, 2015·Critical Reviews in Eukaryotic Gene Expression·Peifu TangLihai Zhang
Aug 4, 2016·Molecular Medicine Reports·Kaixiu FangYingliang Song
Dec 17, 2016·Tissue Engineering. Part B, Reviews·Winston A CamargoJeroen J J P van den Beucken
Sep 15, 2017·Tissue Engineering. Part a·Yuan DengXianqun Fan
Nov 3, 2017·Journal of Molecular Histology·Xing-Kun SunLi-Jun Wang
Nov 4, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jun LinChristian Mehl
Nov 28, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yihua LiuNguyen Tran
Dec 24, 2017·Neuroscience Letters·Ying YeTie Xu
Dec 25, 2017·Brain Research·Hong-Lian ZhangXiao-Mu Wu
Feb 9, 2018·Frontiers in Endocrinology·Carlos MarinKatleen Vandamme
Mar 24, 2018·Journal of Molecular Histology·Radwa Ayman SalahNagwa El-Badri
Citations
Jul 16, 2019·Connective Tissue Research·Yucui JinChangyan Ma
Sep 16, 2019·Journal of Molecular Histology·Doudou LiMeng Cao
Feb 13, 2019·In Vitro Cellular & Developmental Biology. Animal·Xiangdong LiuYingliang Song
Aug 6, 2020·Frontiers in Cell and Developmental Biology·Anastasiia D KurenkovaAndrei S Chagin
Aug 31, 2019·Stem Cells International·Xiaoru XuYingliang Song
Jul 5, 2019·Journal of Molecular Histology·Xijiao YuShanyong Zhang
Aug 13, 2020·International Journal of Molecular Sciences·Qiongyu Lu, Li Zhu
Feb 11, 2021·Journal of Molecular Histology·Zhaoze SunShu Li
Jun 15, 2021·Frontiers in Bioengineering and Biotechnology·Jian LiHong Qiao
Jun 22, 2021·Frontiers in Pharmacology·Weipeng SunZiwei Jiang
Aug 19, 2021·Molecular and Cellular Biochemistry·Ping LiuXing Wei
Oct 15, 2021·Frontiers in Genetics·Chengze WangGuoli Yang