Semaphorin III has been described to function as a guidance molecule directing growing axons to their target. However, its effect on the neuron cell body has not been characterized. Semaphorin III has a highly dynamic expression pattern, which generally corroborates a chemorepellent guidance function, but also suggests additional functions, different from axon guidance. A number of studies show that some sensory neurons are eliminated, while their axons are still pathfinding. In this study we have investigated whether Semaphorin III also influences the survival of sensory neurons. We here present evidence that Semaphorin III can function in vitro as selective death factor for NGF-dependent sensory neurons. Semaphorin III induces a type of cell death that is characterized by slow onset, cell body shrinking, nuclear condensation, and TUNEL-positive staining of dying neurons. These are all hallmarks of neuronal apoptosis. We also show evidence that neurons can modulate the response to Semaphorin III. The novel function described here may also be relevant in vivo, contributing to active elimination of neurons during development or after injury.
Purification and characterization of a trophic factor for embryonic peripheral neurons: comparison with fibroblast growth factors
Development of sympathetic neurons in compartmentalized cultures. Il Local control of neurite growth by nerve growth factor
Development of sympathetic neurons in compartmentalized cultures. II. Local control of neurite survival by nerve growth factor
The death domain motif found in Fas (Apo-1) and TNF receptor is present in proteins involved in apoptosis and axonal guidance
Murine semaphorin D/collapsin is a member of a diverse gene family and creates domains inhibitory for axonal extension
Semaphorin III can function as a selective chemorepellent to pattern sensory projections in the spinal cord
Dorsal root ganglion cell death and surviving cell numbers in relation to the development of sensory innervation in the rat hindlimb
Inhibition of lysophosphatidate- and thrombin-induced neurite retraction and neuronal cell rounding by ADP ribosylation of the small GTP-binding protein Rho
Neurites can remain viable after destruction of the neuronal soma by programmed cell death (apoptosis)
Expression of the UNC-5 guidance receptor in the touch neurons of C. elegans steers their axons dorsally
The guidance molecule semaphorin III is expressed in regions of spinal cord and periphery avoided by growing sensory axons
Developmentally regulated expression of a cell surface protein, neuropilin, in the mouse nervous system
The sensory innervation of the mouse spinal cord may be patterned by differential expression of and differential responsiveness to semaphorins
Anatomy of rat semaphorin III/collapsin-1 mRNA expression and relationship to developing nerve tracts during neuroembryogenesis
Bax deletion further orders the cell death pathway in cerebellar granule cells and suggests a caspase-independent pathway to cell death
A 70 amino acid region within the semaphorin domain activates specific cellular response of semaphorin family members
Secreted chick semaphorins bind recombinant neuropilin with similar affinities but bind different subsets of neurons in situ
Neuropilin-semaphorin III/D-mediated chemorepulsive signals play a crucial role in peripheral nerve projection in mice
Anatomical distribution of the chemorepellent semaphorin III/collapsin-1 in the adult rat and human brain: predominant expression in structures of the olfactory-hippocampal pathway and the motor system
Transition from caspase-dependent to caspase-independent mechanisms at the onset of apoptotic execution
Efficient nuclear targeting of granzyme B and the nuclear consequences of apoptosis induced by granzyme B and perforin are caspase-dependent, but cell death is caspase-independent.
Expression of the gene encoding the chemorepellent semaphorin III is induced in the fibroblast component of neural scar tissue formed following injuries of adult but not neonatal CNS
Neuropilin 1 directly interacts with Fer kinase to mediate semaphorin 3A-induced death of cortical neurons.
Semaphorin 3A contributes to distal pulmonary epithelial cell differentiation and lung morphogenesis.
Inhibition of lung cancer cell growth and induction of apoptosis after reexpression of 3p21.3 candidate tumor suppressor gene SEMA3B
Semaphorin 3B (SEMA3B) induces apoptosis in lung and breast cancer, whereas VEGF165 antagonizes this effect
Upregulation of Semaphorin 3A and the associated biochemical and cellular events in a rat model of retinal detachment
Differential MAP kinases activation during semaphorin3A-induced repulsion or apoptosis of neural progenitor cells
Conditional inactivation of the NBS1 gene in the mouse central nervous system leads to neurodegeneration and disorganization of the visual system
Sustained up-regulation of semaphorin 3A, Neuropilin1, and doublecortin expression in ischemic mouse brain during long-term recovery
Sema4D deficiency results in an increase in the number of oligodendrocytes in healthy and injured mouse brains
Neuroprotective effects of vascular endothelial growth factor (VEGF) upon dopaminergic neurons in a rat model of Parkinson's disease
Semaphorin 3C preserves survival and induces neuritogenesis of cerebellar granule neurons in culture
Semaphorin 3A and neurotrophins: a balance between apoptosis and survival signaling in embryonic DRG neurons
Semaphorin 3A-Neuropilin-1 signaling regulates peripheral axon fasciculation and pathfinding but not developmental cell death patterns
Changes in expression of Class 3 Semaphorins and their receptors during development of the rat retina and superior colliculus
The interrelations between malfunctioning DNA damage response (DDR) and the functionality of the neuro-glio-vascular unit
Differential expression of collapsin response mediator proteins (CRMP/ULIP) in subsets of oligodendrocytes in the postnatal rodent brain
Neurovascular congruence results from a shared patterning mechanism that utilizes Semaphorin3A and Neuropilin-1
Epigenetic regulation in gallbladder cancer: Promoter methylation profiling as emergent novel biomarkers
Microglia and interleukin-1β in ischemic retinopathy elicit microvascular degeneration through neuronal semaphorin-3A
Semaphorin/neuropilin signaling influences the positioning of migratory neural crest cells within the hindbrain region of the chick
Navigating their way to the clinic: emerging roles for axon guidance molecules in neurological disorders and injury
Expression of Semaphorins, Neuropilins, VEGF, and Tenascins in Rat and Human Primary Sensory Neurons after a Dorsal Root Injury
The orthologous human and murine semaphorin 6A-1 proteins (SEMA6A-1/Sema6A-1) bind to the enabled/vasodilator-stimulated phosphoprotein-like protein (EVL) via a novel carboxyl-terminal zyxin-like domain
The effects of voluntary complex and regular wheel running exercises on the levels of 8-oxoguanine DNA glycosylase, semaphorin 3B, H2O2, and apoptosis in the hippocampus of diabetic rats.
Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis
Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.