Semaphorin3A facilitates axonal transport through a local calcium signaling and tetrodotoxin-sensitive voltage-gated sodium channels

Biochemical and Biophysical Research Communications
Masayuki YamaneYoshio Goshima

Abstract

Semaphorin3A (Sema3A), a secreted factor that navigates axons and dendrites of developing neurons, facilitates axonal transport. However, little is known about the mechanism underlying Sema3A-induced facilitation and its functional implications. Here we show that Sema3A induces facilitation of axonal transport via local calcium signaling in growth cone. The facilitation of axonal transport was blocked by inhibitors of voltage-gated sodium channels (tetrodotoxin, TTX), L-type voltage-gated calcium channel, and ryanodine receptor (RyR). Sema3A evoked intracellular Ca(2+) elevation in growth cone by local application of Sema3A to growth cone. Sema3A also activated RyR in growth cone as well as cell body. Notably, TTX suppressed Sema3A-induced RyR activation in cell body but not in growth cone. Our results identify a novel mechanism of Sema3A-induced axonal transport, and further suggest that Sema3A-induced local calcium signaling in growth cone is propagated to cell body in a TTX-sensitive manner.

References

Aug 11, 1998·Neuron·M J Berridge
Oct 16, 1999·Cell·T TakahashiS M Strittmatter
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·O BeharC J Woolf
Feb 19, 2000·Current Opinion in Neurobiology·J A Raper
Aug 10, 2000·Journal of Neurobiology·F NakamuraS M Strittmatter
Oct 18, 2002·Nature·Robert BlumArthur Konnerth
Jun 9, 2004·Trends in Cell Biology·John Henley, Mu-ming Poo
Jul 9, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chanxia LiYoshio Goshima
Feb 16, 2005·Nature Reviews. Neuroscience·Nobutaka Hirokawa, Reiko Takemura
Aug 3, 2005·Nature Reviews. Neuroscience·Larry S ZweifelDavid D Ginty
Dec 3, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Cecilia Hidalgo
Mar 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Asa MoritaYoshio Goshima
Nov 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Naoya YamashitaYoshio Goshima
Jun 10, 2008·Nature Neuroscience·Makoto NishiyamaKyonsoo Hong
Feb 26, 2011·Current Opinion in Neurobiology·Toh Hean Ch'ng, Kelsey C Martin
May 24, 2011·Nature Cell Biology·Makoto NishiyamaKyonsoo Hong

❮ Previous
Next ❯

Citations

Dec 18, 2013·Cellular and Molecular Neurobiology·Andrius KaselisSaulius Šatkauskas
Jun 28, 2014·Trends in Neurosciences·Daniel J SutherlandGeoffrey J Goodhill
Feb 24, 2016·Frontiers in Synaptic Neuroscience·Oleksandr YagenskyJohn Jia En Chua
Feb 28, 2016·Journal of Neuropathology and Experimental Neurology·Sonja KörnerSusanne Petri
Jul 9, 2016·Cell Adhesion & Migration·Yoshio GoshimaYukio Sasaki
Mar 4, 2017·Journal of Cell Science·Masayuki YamaneYoshio Goshima
Mar 6, 2016·Journal of Cell Science·Naoya YamashitaYoshio Goshima
Mar 14, 2020·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroki TanakaShin Takagi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Axon Guidance

Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.