Semen characteristics after overnight shipping: preservation of sperm concentrations, HspA2 ratios, CK activity, cytoplasmic retention, chromatin maturity, DNA integrity, and sperm shape

Journal of Andrology
Gabor HuszarErtug Kovanci

Abstract

We tested several approaches that can be used to preserve sperm attributes and the objective biochemical markers of sperm maturity and function for assessment in a remote centralized laboratory after overnight shipping of semen samples. Addition of phenyl-methyl-sulfonyl-fluoride (PMSF) to a final concentration of 20 microg/mL semen at 4 degrees C has preserved sperm concentrations and HspA2 isoform ratios, even at room temperature, simulating a shipping delay in moderate ambient temperatures. Regarding the attributes of individual spermatozoa, the patterns of CK-immunocytochemistry (demonstrates cytoplasmic retention in diminished-maturity spermatozoa); aniline blue staining pattern (tests chromatin maturity); sperm shape assessed by both Kruger strict morphology and computer assisted morphometry; and sperm DNA integrity, as tested by DNA nick translation, all remained unchanged. Thus, the PMSF-4 degrees C conditions preserved sperm concentrations and the cytoplasmic and nuclear biomarkers of sperm cellular maturity and function for next-day analysis. This shipping method will facilitate the early detection of subtle changes in semen quality that can affect sperm function, even when there has been no decline in sperm concentra...Continue Reading

References

Sep 1, 1982·Journal of Reproduction and Fertility·R V Hyne, D L Garbers
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Citations

Dec 1, 2018·Systems Biology in Reproductive Medicine·Giulia CollodelElena Moretti
Sep 1, 2005·International Journal of Andrology·Joseph JonckheereTrevor G Cooper
Nov 10, 2014·Journal of Assisted Reproduction and Genetics·Leyla SatiGabor Huszar
Jan 30, 2010·Asian Journal of Andrology·Rupert P Amann
Jul 19, 2012·International Journal of Andrology·M H Nasr-EsfahaniM Tavalaee
Jul 22, 2014·Molecular Reproduction and Development·Afsoon Atrian AfiyaniMohammad Hossein Nasr-Esfahani

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