Semen-specific miRNAs: Suitable for the distinction of infertile semen in the body fluid identification?

Forensic Science International. Genetics
Huan TianWeibo Liang

Abstract

Non-protein coding RNA, miRNAs (microRNAs), are a class of promising molecular biomarkers for forensic body fluid identification (BFI) as their small size and tissue-specific expression manners. A number of studies have shown that semen can be distinguished from forensic-related body fluids (such as menstrual blood, venous blood, vaginal fluid, saliva, etc.) using semen-specific miRNAs through microassay screening and RT-qRCR. Infertility is becoming a global health problem, affecting 10%-15% of couples worldwide, and half of the cases are the result of male factors (Lian et al., 2009 [1]). Forensic researchers have to consider the impact of semen infertility on semen identification with a high incidence of infertility. In the present study, normal semen (NS) and four other types of infertile semen samples, including asthenospermia (AS), oligospermia (OS), azoospermia (AZ), oligospermia and asthenospermia (OSAS) semen, were collected. The expression levels of a set of semen-specific miRNA markers (miR-10a, miR-10b, miR-135a, miR-135b, miR-888 and miR-891a) were evaluated using a real-time quantitative PCR technique with a specific fluorescence-labelled TaqMan probe. The results showed the significantly high expression of these ...Continue Reading

Citations

Jun 12, 2019·Journal of Forensic Sciences·Tiffany R LayneSarah J Seashols-Williams
Feb 8, 2020·Andrologia·Zohreh HeidaryHamid Reza Khorram Khorshid
Nov 7, 2019·The American Journal of Forensic Medicine and Pathology·Papanu Suttipasit
Apr 24, 2019·Andrologia·Zohreh HeidaryHamid Reza Khorram Khorshid
Nov 18, 2019·International Journal of Legal Medicine·Hongxia HeQifan Sun
May 21, 2019·Journal of Forensic Sciences·Carolyn A LewisSarah J Seashols-Williams
Mar 20, 2020·Journal of Assisted Reproduction and Genetics·Yousef DaneshmandpourEbrahim Sakhinia
Dec 31, 2020·Diagnostics·Anna RocchiIsabella Spinetti
Nov 11, 2020·Cell Proliferation·Luyen Tien VuMinh T N Le
Jan 22, 2021·Animal Reproduction Science·Audra A HuffmeyerRobert K Wayne
Apr 4, 2021·International Journal of Molecular Sciences·Madalina Gabriela BarbuDragos Valentin Predescu
Jul 8, 2021·Electrophoresis·Huan TianWeibo Liang
Aug 7, 2021·Systems Biology in Reproductive Medicine·Yeganeh Rastgar RezaeiRalf Dittrich
Nov 30, 2018·Analytical Chemistry·Bruce R McCordGeorge Duncan
Nov 6, 2021·Andrologia·Reza Asadpour, Ehsan Mofidi Chelan

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