Semi-allogeneic vaccines and tumor-induced immune tolerance.

Journal of Translational Medicine
Jin YuSebastiano Gattoni-Celli

Abstract

Experimental results from studies with inbred mice and their syngeneic tumors indicated that the inoculation of semi-allogeneic cell hybrids (derived from the fusion between syngeneic tumor cells and an allogeneic cell line) protects the animal host from a subsequent lethal challenge with unmodified syngeneic tumor cells. Semi-allogeneic somatic cell hybrids were generated by the fusion of EL-4 T lymphoma cells (H-2b) and BALB/c-derived renal adenocarcinoma RAG cells (H-2d). Cell hybrids were injected intra-peritoneally (i.p.) in C57BL/6 mice (H-2b) before challenging the mice with a tumorigenic dose of EL-4 cells. Semi-allogeneic tumor cell hybrids could not form a tumor in the animal host because they expressed allogeneic determinants (H-2d) and were rejected as a transplant. However, they conferred protection against a tumorigenic challenge of EL-4 cells compared to control mice that were mock-vaccinated with i.p.-injected phosphate-buffered saline (PBS) and in which EL-4 lymphomas grew rapidly to a large size in the peritoneal cavity. Screening of spleen-derived RNA by means of focused microarray technology showed up-regulation of genes involved in the Th-1-type immune response and in the activation of dendritic antigen-pre...Continue Reading

References

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Jan 1, 1970·Progress in Experimental Tumor Research·F M Burnet
Apr 4, 2000·Journal of Immunotherapy·D A NewtonS Gattoni-Celli
Aug 15, 2001·Antimicrobial Agents and Chemotherapy·B Fernández-TorresJ Guarro
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Nov 18, 2004·Immunological Reviews·Mark J SmythYoshihiro Hayakawa
Aug 10, 2007·Journal of Translational Medicine·Jin YuSebastiano Gattoni-Celli

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Citations

Jan 6, 2016·Journal of Translational Medicine·Mark S KindySebastiano Gattoni-Celli

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Methods Mentioned

BETA
PCR
fluorescence-activated cell sorting

Software Mentioned

GraphPad
GraphPad Prism

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