Semi-anaerobic growth conditions are favoured by some Escherichia coli strains during heterologous expression of some archaeal proteins.

Molecular Biology Reports
Volkan Demir, H B Dincturk

Abstract

Host cell physiology is known to play a crucial role in the expression of foreign genes in heterologous systems. Expression of archaeal genes in anaerobic or semi-anaerobic growth conditions of E. coli has been previously reported to be a means of improving solubility of some proteins. Here, we report that some of the Rosetta strains of E. coli, which harbour the rare tRNA genes for the expression of archaeal genes, favour semi-anaerobic conditions for the expression of putative FMN binding domain of glutamate synthase from Methanocaldococcus jannaschii at low inducer concentrations.

References

Mar 10, 2000·Biochemical and Biophysical Research Communications·J B BroderickW E Broderick
Mar 20, 2001·Molecular Biology Reports·H B Dincturk, D B Knaff
Apr 23, 2002·The Journal of Biological Chemistry·Robert H H van den HeuvelAndrea Mattevi
Aug 29, 2003·Journal of Biochemistry·Yasuhiro Kashima, Kazuhiko Ishikawa
Aug 23, 1985·Science·H W Jannasch, M J Mottl

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