Semi-automated evaluation of Ki-67 index in invasive ductal carcinoma of the breast

Oncology Letters
Cristian SuciuMarius Raica

Abstract

A significant factor that affects the value of the Ki-67 proliferation index (IK) is the interpretation and implementation approach. This method is based on visual or automated methods to count tumor nuclei labeled with Ki-67 antigen, and is prone to errors. Detection of Ki-67 is a useful tool in breast cancer and contributes to its molecular classification. The current study proposes a method for the quantification of Ki-67-positive tumor nuclei, which allows for the determination of the exact IK value that is required for tumor stratification based on the proliferation rate. The IK was assessed in 81 successive cases of diagnosed invasive ductal breast carcinoma using a semi-automated method that accurately identifies positive tumor cell nuclei. This method prevents the inclusion of other possible positive cells, including lymphoid, normal epithelia and hyperplastic. In small specimens with increased cell density, where the nucleus/cytoplasm ratio is markedly in favor of the nucleus and the distance between nuclei is small, the method allows precise quantification of the nuclei, even when the limits between nuclei are difficult to identify. In addition, images may be stored in a database, including the assessments, and easily...Continue Reading

References

Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·J GerdesH Stein
Aug 30, 2000·Nature·C M PerouD Botstein
Jan 5, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·R L CampD L Rimm
May 25, 2001·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·R S RampaulUNKNOWN Nottingham Breast Team
Sep 5, 2001·Histopathology·B Dunne, J J Going
Jun 8, 2002·Applied Immunohistochemistry & Molecular Morphology : AIMM·Daniel A Arber
Jun 15, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kyle A DiVitoRobert L Camp
Nov 25, 2004·The Oncologist·Mary Cianfrocca, Lori J Goldstein
Sep 30, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ander UrruticoecheaMitch Dowsett
Jun 8, 2006·JAMA : the Journal of the American Medical Association·Lisa A CareyRobert C Millikan
Dec 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Antonio C WolffUNKNOWN College of American Pathologists
Dec 14, 2006·Breast Cancer Research : BCR·Mark J BeresfordAndreas Makris
Apr 27, 2007·Virchows Archiv : an International Journal of Pathology·Ossama TawfikCarol J Fabian
Jun 8, 2007·Virchows Archiv : an International Journal of Pathology·Daehoon ParkTorill Sauer
Oct 25, 2007·Cellular and Molecular Life Sciences : CMLS·P B Mullan, R C Millikan
Nov 5, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Giuseppe VialeUNKNOWN Breast International Group Trial 1-98
Mar 26, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Emad A RakhaIan O Ellis
May 14, 2009·Journal of the National Cancer Institute·Maggie C U CheangTorsten O Nielsen
Jun 19, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A GoldhirschUNKNOWN Panel members
Feb 16, 2010·The Lancet Oncology·Rinat YerushalmiKaren A Gelmon
Sep 2, 2010·Breast Cancer Research : BCR·Emad A RakhaIan O Ellis
Jun 29, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A GoldhirschUNKNOWN Panel members
Oct 1, 2011·Journal of the National Cancer Institute·Mitch DowsettUNKNOWN International Ki-67 in Breast Cancer Working Group
Jan 13, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Nov 7, 2012·Japanese Journal of Clinical Oncology·Takeshi Yamaguchi, Hirofumi Mukai

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry

Software Mentioned

Elements
NIS

Related Concepts

Related Feeds

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.