Semi-continuous pilot-scale microbial oil production with Metschnikowia pulcherrima on starch hydrolysate

Biotechnology for Biofuels
Felix AbelnChristopher J Chuck

Abstract

Heterotrophic microbial oils are potentially a more sustainable alternative to vegetable or fossil oils for food and fuel applications. However, as almost all work in the area is conducted on the laboratory scale, such studies carry limited industrial relevance and do not give a clear indication of what is required to produce an actual industrial process. Metschnikowia pulcherrima is a non-pathogenic industrially promising oleaginous yeast which exhibits numerous advantages for cost-effective lipid production, including a wide substrate uptake, antimicrobial activity and fermentation inhibitor tolerance. In this study, M. pulcherrima was fermented in stirred tank reactors of up to 350 L with 250-L working volume in both batch and semi-continuous operation to highlight the potential industrial relevance. Due to being food-grade, suitable for handling at scale and to demonstrate the oligosaccharide uptake capacity of M. pulcherrima, enzyme-hydrolysed starch in the form of glucose syrup was selected as fermentation feedstock. In batch fermentations on the 2-L scale, a lipid concentration of 14.6 g L-1 and productivity of 0.11 g L-1 h-1 were achieved, which was confirmed at 50 L (15.8 g L-1; 0.10 g L-1 h-1). The maximum lipid produ...Continue Reading

References

Jul 10, 2001·Journal of Applied Microbiology·M L StraussP van Rensburg
Oct 13, 2001·Journal of Industrial Microbiology & Biotechnology·C R ZastrowB U Stambuk
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jul 8, 2005·Applied Microbiology and Biotechnology·F R Schmidt
Apr 8, 2010·Bioresource Technology·Feiyan XueTianwei Tan
Jan 22, 2014·Journal of Applied Microbiology·L OroF Comitini
Aug 31, 2014·Biotechnology Advances·Irnayuli R SitepuKyria L Boundy-Mills
Mar 4, 2015·Metabolic Engineering·Kangjian QiaoGregory Stephanopoulos

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
genetic modification

Software Mentioned

GIMP
Image J

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.