Semi-Preparative Separation of 10 Caffeoylquinic Acid Derivatives Using High Speed Counter-Current Chromatogaphy Combined with Semi-Preparative HPLC from the Roots of Burdock (Arctium lappa L.)

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Zhenjia ZhengXuguang Qiao

Abstract

Burdock roots are healthy dietary supplements and a kind of famous traditional Chinese medicine, which contains large amounts of caffeoylquinic acid derivatives. However, little research has been reported on the preparative separation of these compounds from burdock roots. In the present study, a combinative method of HSCCC and semi-preparative HPLC was developed for the semi-preparative separation of caffeoylquinic acid derivatives from the burdock roots. The ethyl acetate extract of burdock roots was first fractionated by MCI macroporous resin chromatography and give three fractions (Fr. 1-3) from the elution of 40% methanol. Then, these three fractions (120 mg) were separately subjected to HSCCC for purification with the solvent system composed of petroleum ether-ethyl acetate-methanol-water at different volume ratios, and the mixtures were further purified by semi-preparative HPLC. As a result, a total of eight known caffeoylquinic acid derivatives including 3-O-caffeoylquinic acid (32.7 mg, 95.7%), 1,5-O- dicaffeoylquinic acid (4.3 mg, 97.2%), 3-O-caffeoylquinic acid methyl ester (12.1 mg, 93.2%), 1,3-O-dicaffeoylquinic acid (42.9 mg, 91.1%), 1,5-O-dicaffeoyl-3-O-(4-maloyl)-quinic acid (4.3 mg, 84.5%), 4,5-O-dicaffeoylquin...Continue Reading

References

Jan 1, 1996·The American Journal of Chinese Medicine·C C LinT Ujiie
Jul 14, 2001·Fitoterapia·A CarnatJ L Lamaison
Nov 1, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Victoria Castel, Elena Grau
Apr 23, 2009·Natural Product Research·Xian-Feng ZhuRamond Lo
Oct 6, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hualiang ZengXiaoqing Chen

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Citations

Sep 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Myeong Il KimChul Young Kim
Sep 19, 2018·International Journal of Molecular Medicine·Nuramina MamatHaji Akber Aisa

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Methods Mentioned

BETA
column chromatography

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