Semi-purification of the immunoglobulin E-sweat antigen acting on mast cells and basophils in atopic dermatitis

Experimental Dermatology
A TanakaMichihiro Hide

Abstract

Sweating aggravates the symptoms of atopic dermatitis (AD). We have recently reported positive skin reactions and histamine release from basophils in response to autologous sweat in patients with AD. To characterize the biochemical and immunological properties of the substance in sweat that evokes histamine release and to study the usability of the basophil-histamine release test with the sweat antigen for AD. Sweat collected from healthy volunteers was purified using chromatographies. Serum immunoglobulin (Ig)E of four patients with AD were purified using an affinity-chromatography column with anti-IgE antibodies. The amount of semi-purified sweat antigen (138 ng protein/ml) that induced a half-maximum reaction of basophils of a patient with AD was utilized for the basophil histamine release test. The involvement of specific IgE and high-affinity IgE receptor (FcepsilonRI) in the reactions was examined using basophils of healthy volunteers, a human mast cell line (LAD2), and a rat basophilic leukemia cell line transfected with human alpha-subunit of FcepsilonRI (RBL-48). The semi-purified sweat antigen induced histamine release from the basophils of 47 of 61 (74.6%) patients with AD and four of 46 (8.7%) healthy controls. Both...Continue Reading

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Citations

Aug 7, 2009·Current Allergy and Asthma Reports·Tastuya HorikawaChikako Nishigori
Mar 14, 2012·The Journal of Allergy and Clinical Immunology·Ting Seng TangHywel C Williams
Nov 7, 2015·Biochemical and Biophysical Research Communications·Kaori IshiiMichihiro Hide
Sep 27, 2008·The Journal of Investigative Dermatology·Ikue Nemoto-HasebeHiroshi Shimizu
Oct 25, 2008·Journal of Dermatological Science·Hiroaki MatsuoEishin Morita
Jun 29, 2015·Allergology International : Official Journal of the Japanese Society of Allergology·Takanobu KanMichihiro Hide
Oct 25, 2013·Allergology International : Official Journal of the Japanese Society of Allergology·Hiroaki MatsuoMichihiro Hide
Aug 22, 2013·Allergology International : Official Journal of the Japanese Society of Allergology·Tomoharu YokoojiHiroaki Matsuo
Mar 3, 2011·Allergology International : Official Journal of the Japanese Society of Allergology·Takeshi KozaruChikako Nishigori
Oct 25, 2012·Allergology International : Official Journal of the Japanese Society of Allergology·Toshinori BitoYoshiki Tokura
Sep 18, 2008·Allergology International : Official Journal of the Japanese Society of Allergology·Hidenori SuzukiMichihiro Hide
Jan 25, 2014·Allergology International : Official Journal of the Japanese Society of Allergology·Makiko HiragunMichihiro Hide
Jun 4, 2013·The Journal of Allergy and Clinical Immunology·Takaaki HiragunMichihiro Hide
Feb 5, 2008·Journal of Toxicology and Environmental Health. Part a·Nai-Yu XuJian Tong
Jul 2, 2011·Bulletin of Environmental Contamination and Toxicology·Muthukannan Satheesh KumarMuthukumaran Gunasekaran
Aug 11, 2020·Clinical and Translational Allergy·Fariza Mishaal Saiema BadloeInge Kortekaas Krohn
Jun 10, 2020·Clinical and Translational Allergy·Sabine AltrichterMarcus Maurer
Nov 11, 2021·The Journal of Dermatology·Rie NakamuraMichihiro Hide

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