PMID: 41707Jan 1, 1979

Semiautomated enzymic microassay for plasma L-alanine

Enzyme
A J StahlJ L Schlienger

Abstract

Plasma L-alanine is deaminated by bacterial alanine-dehydrogenase; the resulting ammonia is dialyzed out and measured by use of a continuous flow phenol-hypochlorite colorimetric microassay. Concentrations in the range of 10-1,000 mumol alanine/l can be determined in a 50-microliter sample. The optimal conditions for the assay are specified. Study of the analytical qualities of the technique shows high specificity, good reproducibility , and a detection limit of 6 mumol/l. Usual values in human plasma from arterial or venous blood are respectively 296 +/- 166 and 376 +/- 214 mumol alanine/l (x +/- 2 SD). The usual values in rats are close to those found in man.

References

Jan 10, 2019·Bioscience, Biotechnology, and Biochemistry·Yasuhisa Asano

Related Concepts

Human plasma
Phenol
Venous Blood Substance
Portion of Venous Blood
NADH
Abufne
Blood Ammonia Measurement
Ammonia Inhalants
Fasting
Phenols

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