Seminal exosomes induce interleukin-6 and interleukin-8 secretion by human endometrial stromal cells

European Journal of Obstetrics, Gynecology, and Reproductive Biology
Shahrokh PaktinatHamid Nazarian

Abstract

Exosomes are extracellular microvesicles that participate in intercellular communication. Seminal plasma (SP) contains very large amounts of exosomes which are deposited in female genital tract after insemination. Although the response of vaginal cells to seminal exosomes (SE) is recently being elucidated, the interaction of uterine cells with SE is still unknown. Here, we aimed to evaluate the effect of SE on cytokine secretion by human endometrial stromal cells (eSC). Exosomes were isolated from the semen samples of healthy men with proven fertility and characterized using common exosome characterization methods. Human eSC were isolated from endometrial biopsies obtained from healthy premenopausal women. For exosome internalization analysis, SE were labeled with PKH67 green fluorescent dye and incubated with the cells. For investigating the effect of SE on cytokine secretion of eSC, we measured levels of interleukin (IL)-6, IL-8, IL-10, IL-1α, and leukemia inhibitory factor (LIF) in the culture supernatants of control and experimental groups by enzyme-linked immunosorbent assay (ELISA) after 24 h of incubation. Our results demonstrated that SE are internalized by eSC and subsequently induce them to produce IL-6 and IL-8, the ...Continue Reading

Citations

Jul 9, 2020·Reproductive Sciences·Marina NikolaevaGennady Sukhikh
Oct 17, 2020·American Journal of Reproductive Immunology : AJRI·Anuradha MishraDeepak Modi
May 29, 2021·Frontiers in Immunology·Kunfeng BaiPhilip C N Chiu
May 26, 2021·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Shahrokh PaktinatHamid Nazarian
Jun 22, 2021·Frontiers in Endocrinology·Nan-Xing Jiang, Xue-Lian Li
Jul 27, 2021·Expert Review of Molecular Diagnostics·Raheleh HeydariAnna Meyfour
Dec 4, 2021·Frontiers in Physiology·Barbara FraserRobert J Aitken

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