Seminiferous tubule fluid secretion is a Sertoli cell microtubule-dependent process inhibited by 2,5-hexanedione exposure

Toxicology and Applied Pharmacology
J H RichburgK Boekelheide


Mammalian Sertoli cells are responsible for the formation and secretion of seminiferous tubule fluid (STF) which provides the nutritional and hormonal microenvironment necessary for spermatogenesis. Exposure of rats to 2,5-hexanedione (2,5-HD) results in testicular injury characterized by a decrease of STF secretion which immediately precedes bulk germ cell necrosis. The earliest biochemical change in 2,5-HD-exposed rats is an alteration in testicular microtubule assembly kinetics. In this study, we investigate the relationship between microtubule-dependent processes and STF secretion in adult Sprague-Dawley CD rats by exposing seminiferous tubules to two types of toxicants: (1) those which alter microtubules (colchicine and 2,5-HD) and (2) an inhibitor of protein secretion (brefeldin A, [BFA]). Secretion of STF is quantitated by monitoring the rate of transport of a microinjected oil droplet in the lumen of isolated seminiferous tubules using time-lapse stereoscopic microscopy. The rate of oil droplet transport in seminiferous tubules isolated from testis pretreated in vivo for 2 hr with colchicine (40 micrograms/testis) was significantly decreased. Exposure of isolated seminiferous tubules to BFA (10 micrograms/ml) for 40 min...Continue Reading


Oct 24, 2000·Proceedings of the Society for Experimental Biology and Medicine·K BoekelheideHeidi A Schoenfeld
Apr 25, 2007·Journal of Occupational and Environmental Hygiene·Michael P WilsonAlan E Hubbard
Dec 10, 2002·Annual Review of Pharmacology and Toxicology·K BoekelheideStephanie Thompson
Feb 7, 2016·Environmental Science and Pollution Research International·Shuya ZhangXin Cao
Mar 1, 1997·Toxicology and Applied Pharmacology·S D CohenJ A Hinson
Jun 9, 2005·Reproductive Toxicology·Paul E KihaileJunichi Misumi
Apr 12, 2017·Birth Defects Research·Noriko NakamuraDeborah K Hansen
May 20, 2004·Reproductive Medicine and Biology·Kazuo AokiMasanobu Kudo

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