PMID: 11918546Mar 29, 2002Paper

Semiquantitative measurement of IgG subclasses and IgM of platelet-specific antibodies in a glycoprotein-specific platelet-antigen capture assay

British Journal of Haematology
Reinhard KelschWalter Sibrowski

Abstract

The detection of platelet-specific antibodies is of high clinical interest in diseases with immune thrombocytopenia. The glycoprotein-specific platelet-antigen capture (PAC)-assay developed in this study is especially suited to the differentiation of platelet-specific immunoglobulin (Ig) G subclasses and the determination of platelet-specific IgM in serum or on platelets. The problems with unspecific signals or low sensitivity usually seen with the detector antibodies available are effectively overcome, as unbound detector antibodies are removed at an early stage in the assay. We investigated 14 maternal alloantisera from cases of neonatal alloimmune thrombocytopenia (NAITP) and six sera from patients with autoimmune thrombocytopenic purpura (AITP). In NAITP sera, we found IgG1 alone in 57%, IgG1 + IgG3 in 21% and IgG1 + IgG2 in 14% of cases. One serum contained IgG1 + IgG2 + IgG3. In AITP, three out of the six sera contained IgG1 alone. One serum contained IgG1 + IgG2. One patient, with highly refractory AITP, had platelet-specific IgG1 + IgG2 + IgG3 in his serum. A patient with AITP in remission and normal platelet counts only showed platelet-bound IgG2. The detection of platelet-specific 'whole IgG' is possible too. However,...Continue Reading

References

Sep 1, 1992·Transfusion Medicine·V Kiefel
Apr 1, 1990·Baillière's Clinical Haematology·C P Engelfriet, W H Ouwehand
Jun 1, 1986·American Journal of Obstetrics and Gynecology·M M TaslimiJ V Dacus
Apr 15, 1985·American Journal of Obstetrics and Gynecology·J ParinaudG Pontonnier
Jun 1, 1980·British Journal of Haematology·A E von dem BorneC P Engelfriet
Dec 1, 1996·British Journal of Haematology·R McMillanS Goodnight

❮ Previous
Next ❯

Citations

Sep 20, 2012·Clinical & Developmental Immunology·Eduardo Luzía FrançaAdenilda Cristina Honorio-França
Mar 17, 2020·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Simon KingsleyDaniel Dolly
Jul 30, 2016·Scandinavian Journal of Immunology·E G de SouzaA C Honorio-França

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.