PMID: 7543991May 5, 1995Paper

Sendai virosomal infusion of an adeno-associated virus-derived construct containing neuropeptide Y into primary rat brain cultures

Neuroscience Letters
P WuE M Meyer

Abstract

A novel neuronal gene-delivery system was investigated in primary neuron-enriched cultures with respect to driving the expression of neuropeptide Y (NPY). This delivery system consists of an adeno-associated virus-derived (AAV) plasmid, pJDT95npy, encapsulated in reconstituted Sendai virosomes. pJDT95npy contains full length rat NPY cDNA inserted downstream from the P40 promoter in a cap-gene deleted AAV-derived construct. The rep-sequences under control of the P5 and P19 promoters are intact. Virosomally encapsulated pJDT95npy drove the expression of NPY mRNAs, predominantly by P40. Total cellular NPY immunoreactivity and release in the presence of depolarization increased following pJDT95npy-transfection. Neither empty virosomes nor virosomes containing pJDT95 affected NPY mRNA expression or immunoreactivity. This study demonstrates that an AAV-derived plasmid can drive exogenous gene expression in intact neurons after infusion by Sendai virosomes.

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R M KotinK I Berns
Sep 1, 1990·Microbiological Reviews·K I Berns
Jan 1, 1990·Archives of Virology·C S ChoiT W Molitor
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B G Stanley, S F Leibowitz
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P L Hermonat, N Muzyczka
Aug 26, 1983·Science·Y S AllenJ M Polak
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K Tatemoto
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·M G KaplittD W Pfaff
Oct 1, 1993·Neurochemical Research·C M de FiebreE M Meyer
Jul 16, 1993·Brain Research. Developmental Brain Research·J J PoulakosE M Meyer

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Citations

Oct 1, 1996·Current Opinion in Neurobiology·R S Slack, F D Miller
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·V J DzauY Kaneda

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