Sendai virus-based liposomes enable targeted cytosolic delivery of nanoparticles in brain tumor-derived cells.

Journal of Nanobiotechnology
Veronica DuduMaribel Vazquez

Abstract

Nanotechnology-based bioassays that detect the presence and/or absence of a combination of cell markers are increasingly used to identify stem or progenitor cells, assess cell heterogeneity, and evaluate tumor malignancy and/or chemoresistance. Delivery methods that enable nanoparticles to rapidly detect emerging, intracellular markers within cell clusters of biopsies will greatly aid in tumor characterization, analysis of functional state and development of treatment regimens. Experiments utilized the Sendai virus to achieve in vitro, cytosolic delivery of Quantum dots in cells cultured from Human brain tumors. Using fluorescence microscopy and Transmission Electron Microscopy, in vitro experiments illustrated that these virus-based liposomes decreased the amount of non-specifically endocytosed nanoparticles by 50% in the Human glioblastoma and medulloblastoma samples studied. Significantly, virus-based liposome delivery also facilitated targeted binding of Quantum dots to cytosolic Epidermal Growth Factor Receptor within cultured cells, focal to the early detection and characterization of malignant brain tumors. These findings are the first to utilize the Sendai virus to achieve cytosolic, targeted intracellular binding of Qd...Continue Reading

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Citations

Aug 16, 2015·Targeted Oncology·Francesca PistollatoMaurizio Battino
Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Hongmei XiaYinxiang Xu
Nov 26, 2013·Cellular and Molecular Bioengineering·Veronica DuduMaribel Vazquez
Apr 29, 2015·World Medical & Health Policy·Shannon GibsonTrudo Lemmens
Jan 6, 2021·Topics in Current Chemistry·Sueden O SouzaGoreti Pereira
Jan 7, 2022·Biomaterials Science·Ruiqi LiJinghua Ren

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Methods Mentioned

BETA
confocal microscopy
biopsy

Software Mentioned

Matlab
NIH Image
NIH Image Software
VR

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