Sendai virus-mediated transduction of mammalian spermatogonial stem cells†

Biology of Reproduction
Satoshi WatanabeTakashi Shinohara

Abstract

Spermatogonial stem cells (SSCs) provide the foundation of spermatogenesis. However, because of their small number and slow self-renewal, transfection of SSCs has met with limited success. Although several viral vectors can infect SSCs, genome integration and an inability to maintain long-term gene expression have hampered studies on SSCs. Here we report successful SSC infection by Sendai virus (SV), an RNA virus in the Paramyxoviridae. The SV efficiently transduced germline stem (GS) cells, cultured spermatogonia with enriched SSC activity, and maintained gene expression for at least 5 months. It also infected freshly isolated SSCs from adult testes. The transfected GS cells reinitiated spermatogenesis following spermatogonial transplantation into seminiferous tubules of infertile mice, suggesting that SV transfection does not interfere with spermatogenesis progression. On the other hand, microinjection of SV into the seminiferous tubules of immature mice transduced SSCs and Sertoli cells, but did not transduce Leydig or peritubular cells by interstitial virus injection. SV-infected hamster GS cells, and freshly isolated rabbit or monkey SSC-like cells were identified following xenogeneic spermatogonial transplantation, sugges...Continue Reading

References

Jan 1, 1978·Advances in Virus Research·N Ishida, M Homma
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M A Markwell, J C Paulson
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Brinster, J W Zimmermann
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Brinster, M R Avarbock
May 30, 1996·Nature·D E ClouthierR L Brinster
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·P PaleseA García-Sastre
Oct 21, 1999·Biology of Reproduction·I DobrinskiR L Brinster
Oct 29, 2000·Advances in Virus Research·L Naldini, I M Verma
Apr 25, 2001·Biology of Reproduction·M NaganoR L Brinster
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ShinoharaR L Brinster
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M NaganoR L Brinster
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mito Kanatsu-ShinoharaTakashi Shinohara
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Masahito IkawaInder M Verma
Jul 24, 2002·FEBS Letters·Makoto NaganoRalph L Brinster
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·F Kent HamraDavid L Garbers
Apr 18, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Jun 24, 2003·The Journal of Biological Chemistry·Satoshi KarasawaAtsushi Miyawaki
Sep 5, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Jan 16, 2004·Gene Therapy·S N WaddingtonM Bitzer
Jun 11, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Oct 1, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
May 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mito Kanatsu-ShinoharaTakashi Shinohara
Oct 13, 2006·Biology of Reproduction·Masutaka TokudaTohru Marunouchi
Feb 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Masanori TakehashiTakashi Shinohara
Dec 21, 2007·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Oct 16, 2008·Clinical Microbiology Reviews·Shyam Daya, Kenneth I Berns
Oct 20, 2009·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Noemi FusakiMamoru Hasegawa
Nov 26, 2009·PloS One·Hiroko MorimotoTakashi Shinohara
Sep 17, 2010·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Jan 16, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yoshikazu YonemitsuYoshihiko Maehara
Jun 12, 2013·Cell Stem Cell·Hiroko MorimotoTakashi Shinohara
Oct 9, 2013·Annual Review of Cell and Developmental Biology·Mito Kanatsu-Shinohara, Takashi Shinohara
Sep 12, 2014·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Dec 2, 2014·Nature Biotechnology·Thorsten M SchlaegerGeorge Q Daley
Nov 27, 2015·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Aug 10, 2016·Developmental Cell·Mito Kanatsu-ShinoharaTakashi Shinohara
Sep 9, 2016·Molecular Therapy. Methods & Clinical Development·Arnold ParkBenhur Lee
Dec 23, 2016·Genes & Development·Mito Kanatsu-ShinoharaTakashi Shinohara

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