Senescence-inducible LEC2 enhances triacylglycerol accumulation in leaves without negatively affecting plant growth

Plant Biotechnology Journal
Hyun Uk KimJong Bum Kim

Abstract

The synthesis of fatty acids and glycerolipids in wild-type Arabidopsis leaves does not typically lead to strong triacylglycerol (TAG) accumulation. LEAFY COTYLEDON2 (LEC2) is a master regulator of seed maturation and oil accumulation in seeds. Constitutive ectopic LEC2 expression causes somatic embryogenesis and defects in seedling growth. Here, we report that senescence-inducible LEC2 expression caused a threefold increase in TAG levels in transgenic leaves compared with that in the leaves of wild-type plants. Plant growth was not severely affected by the accumulation the TAG in response to LEC2 expression. The levels of plastid-synthesized lipids, mono- and di-galactosyldiacylglycerol and phosphatidylglycerol were reduced more in senescence-induced LEC2 than in endoplasmic reticulum-synthesized lipids, including phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol. Senescence-induced LEC2 up-regulated the expression of many genes involved in fatty acid and TAG biosynthesis at precise times in senescent leaves, including WRINKLED1 (WRI1), which encodes a fatty acid transcription factor. The expressions of glycerol-3-phosphate dehydrogenase 1 and phospholipid:diacylglycerol 2 were increased in the transgenic ...Continue Reading

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Citations

Feb 5, 2016·Annual Review of Plant Biology·Changcheng Xu, John Shanklin
May 5, 2016·The Plant Journal : for Cell and Molecular Biology·Chloé GuerinStéphane Dussert
Aug 25, 2016·Critical Reviews in Biotechnology·Sehrish MananJian Zhao
Jan 14, 2017·Plant Biotechnology Journal·Olga YurchenkoJohn M Dyer
Jan 6, 2018·International Journal of Molecular Sciences·Xia-Jie JiRun-Zhi Li
Oct 10, 2018·International Journal of Molecular Sciences·Li WangAomin Bao
Nov 30, 2019·Plant Science : an International Journal of Experimental Plant Biology·Yang BaiWenhua Zhang
Feb 9, 2021·Bioresource Technology·Ramkrishna SinghVijay Singh
Mar 2, 2019·Progress in Lipid Research·Thomas VanherckeSurinder P Singh
Jul 9, 2020·Progress in Lipid Research·Sandra M CorreaYariv Brotman
Aug 6, 2017·Progress in Lipid Research·Lu-Lu XueJian-Guo Jiang

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