Senescence marker protein-30 as a novel antiaging molecule

Annals of the New York Academy of Sciences
Dongyun FengNaoki Maruyama

Abstract

Senescence marker protein-30 (SMP30), composed of 299 amino acids, has an approximate molecular mass of 32-34 kDa and has a pI 4.9 in charge. The amino acid alignment from various animal species revealed a highly conserved structure. SMP30 has an enzyme activity hydrolyzing sarin, soman, and tabun, known as lethal toxic nerve chemicals. We analyzed the organophosphatase activity of SMP30 using DFP as a substrate. This DFPase activity is revealed in a dose-dependent manner in the presence of magnesium ions. We investigated the intracellular localization of SMP30. It is localized in both the cytoplasm and nucleus. To confirm the presence of SMP30 in the nucleus, we prepared nuclear and cytoplasmic extracts from isolated cultured hepatocytes. Western blotting showed that SMP30 was detected in both extracts. Because the expression is reduced by carbon tetrachloride, one can speculate that the expression is modulated by oxidative stress increased with aging.

References

Oct 1, 1998·Biochemical and Biophysical Research Communications·T FujitaN Maruyama
Jul 27, 1999·Chemico-biological Interactions·S S BilleckeC A Broomfield
May 2, 2000·Journal of Biochemistry·Y Nakajima, S Natori
Jul 27, 2001·Pathology International·T IshigamiN Maruyama
Mar 7, 2003·Bioscience, Biotechnology, and Biochemistry·Akihito IshigamiPrakash C Supakar

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Citations

Nov 29, 2011·Molecular Human Reproduction·Sandra S LaurentinoSílvia Socorro
Jan 20, 2015·Geriatrics & Gerontology International·Rei TakahashiKisaburo Nagata
Apr 7, 2009·Journal of Cellular Biochemistry·Cláudio MaiaSilvia Socorro
Feb 28, 2006·Mechanisms of Ageing and Development·Tae Gen SonJaewon Lee
Nov 9, 2011·Clinics in Geriatric Medicine·Arthur T Fort
Dec 14, 2016·Annual Review of Physiology·Peter J Barnes
Jan 24, 2015·The European Respiratory Journal·Peter J Barnes
Aug 3, 2017·Molecular Medicine Reports·Shuning LiHao Liang

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