Oct 1, 1985

Senescent and quiescent cell inhibitors of DNA synthesis. Membrane-associated proteins

Experimental Cell Research
O M Pereira-SmithJ R Smith

Abstract

Cytoplasts derived from senescent and quiescent human diploid cells inhibit DNA synthesis initiation when fused with cells capable of proliferation. When the cytoplasts were subjected to a variety of conditions (trypsin and cycloheximide treatment and growth on fibronectin), this inhibitory activity was lost, suggesting that the inhibitors involved were proteins associated with the surface membranes of the cells. We have studied the quiescent cell inhibitor in greater detail and determined that surface membrane-enriched preparations isolated from quiescent cells and proteins extracted from these membrane preparations have DNA synthesis-inhibitory activity.

Mentioned in this Paper

Tissue Membrane
Peptide Hydrolases
Transcription Initiation
Trypsin
Cell Division Phases
FN1
Cell Fusion Procedure
DNA Biosynthetic Process
Fibroblasts
DNA Replication

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