Senescent vs. non-senescent cells in the human annulus in vivo: cell harvest with laser capture microdissection and gene expression studies with microarray analysis.

BMC Biotechnology
Helen E GruberEdward N Hanley

Abstract

Senescent cells are well-recognized in the aging/degenerating human disc. Senescent cells are viable, cannot divide, remain metabolically active and accumulate within the disc over time. Molecular analysis of senescent cells in tissue offers a special challenge since there are no cell surface markers for senescence which would let one use fluorescence-activated cell sorting as a method for separating out senescent cells. We employed a novel laser capture microdissection (LCM) design to selectively harvest senescent and non-senescent annulus cells in paraffin-embedded tissue, and compared their gene expression with microarray analysis. LCM was used to separately harvest senescent and non-senescent cells from 11 human annulus specimens. Microarray analysis revealed significant differences in expression levels in senescent cells vs non-senescent cells: 292 genes were upregulated, and 321 downregulated. Genes with established relationships to senescence were found to be significantly upregulated in senescent cells vs. non-senescent cells: p38 (MPAK14), RB-Associated KRAB zinc finger, Discoidin, CUB and LCCL domain, growth arrest and DNA-damage inducible beta, p28ING5, sphingosine-1-phosphate receptor 2 and somatostatin receptor 3; ...Continue Reading

References

Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·G P DimriO Pereira-Smith
Feb 3, 1995·The Journal of Biological Chemistry·S JayadevY A Hannun
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·W R SellersW G Kaelin
Dec 22, 1995·The Journal of Biological Chemistry·M E VenableL M Obeid
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·G KériA Ullrich
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J Campisi
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M Oshimura, J C Barrett
May 23, 1998·Experimental Cell Research·A Evdokiou, P A Cowled
Jul 21, 1998·Annals of the New York Academy of Sciences·E S Gonos
Oct 6, 1999·Current Biology : CB·D N SheltonW D Funk
Feb 12, 2002·The Journal of Biological Chemistry·Steven R SchwarzeDavid F Jarrard
Feb 13, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroaki IwasaFuyuki Ishikawa
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hong ZhangStanley N Cohen
May 17, 2003·Cancer Biology & Therapy·David M ThomasPhilip W Hinds
Mar 1, 1965·Experimental Cell Research·L HAYFLICK
Jan 1, 2004·The Journal of Clinical Investigation·Ittai Ben-Porath, Robert A Weinberg
Mar 4, 2005·The International Journal of Biochemistry & Cell Biology·Ittai Ben-Porath, Robert A Weinberg
Mar 9, 2005·The Journal of Cell Biology·Karin EhrenreiterManuela Baccarini
Apr 6, 2006·The Journal of Bone and Joint Surgery. American Volume·Jeffrey N Katz
Apr 11, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Jun Chen, Michael S Goligorsky
Jun 15, 2006·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·S RobertsS M Eisenstein
Apr 27, 2007·Annals of the New York Academy of Sciences·Andrea B MaierDiana VAN Heemst

❮ Previous
Next ❯

Citations

Mar 8, 2012·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Gabriela CiapettiNicola Baldini
May 23, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·James R Van Brocklyn, Joseph B Williams
Jul 26, 2011·The Spine Journal : Official Journal of the North American Spine Society·Helen E GruberEdward N Hanley
Feb 19, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Nam V VoJames D Kang
Dec 21, 2016·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·Malik GhannamR Shane Tubbs
Jun 13, 2012·Journal of Anatomy·Girish PattappaSibylle Grad
Sep 16, 2016·Frontiers in Aging Neuroscience·Elizabeth P CroweClaudio Torres
Nov 27, 2018·Current Molecular Biology Reports·Prashanti PatilNam Vo
Apr 7, 2018·Spine Surgery and Related Research·Sean M RiderJames D Kang

❮ Previous
Next ❯

Methods Mentioned

BETA
laser capture microdissection
in vitro transcription

Software Mentioned

GCOS Affymetrix GeneChip Operating System
[UNK]
Affymetrix

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
S RobertsS M Eisenstein
European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
Judith Campisi
© 2021 Meta ULC. All rights reserved