Sensing chromosome bi-orientation by spatial separation of aurora B kinase from kinetochore substrates

Science
Dan LiuSusanne M A Lens

Abstract

Successful cell division requires that chromosomes attach to opposite poles of the mitotic spindle (bi-orientation). Aurora B kinase regulates chromosome-spindle attachments by phosphorylating kinetochore substrates that bind microtubules. Centromere tension stabilizes bi-oriented attachments, but how physical forces are translated into signaling at individual centromeres is unknown. Using fluorescence resonance energy transfer-based biosensors to measure localized phosphorylation dynamics in living cells, we found that phosphorylation of an Aurora B substrate at the kinetochore depended on its distance from the kinase at the inner centromere. Furthermore, repositioning Aurora B closer to the kinetochore prevented stabilization of bi-oriented attachments and activated the spindle checkpoint. Thus, centromere tension can be sensed by increased spatial separation of Aurora B from kinetochore substrates, which reduces phosphorylation and stabilizes kinetochore microtubules.

References

Jan 31, 1997·Science·R B Nicklas
Jun 12, 1998·The Journal of Cell Biology·J C WatersE D Salmon
Apr 30, 2003·The Journal of Cell Biology·Claire DitchfieldStephen S Taylor
Feb 10, 2004·Nature Cell Biology·Michael A LampsonTarun M Kapoor
Feb 13, 2004·Developmental Cell·Paul D AndrewsJason R Swedlow
Jun 25, 2004·Nature·Randy Schekman
Jan 10, 2006·Developmental Cell·A Wayne OrrMartin A Schwartz
Sep 13, 2007·Nature Reviews. Molecular Cell Biology·Sandrine RuchaudWilliam C Earnshaw
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·Iain M Cheeseman, Arshad Desai
Aug 5, 2008·Chromosoma·Tomoyuki U Tanaka

❮ Previous
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Citations

Mar 17, 2010·Cellular and Molecular Life Sciences : CMLS·Geert J P L KopsPatrick Meraldi
Mar 26, 2010·Cellular and Molecular Life Sciences : CMLS·Bruce F McEwen, Yimin Dong
Feb 1, 2012·Chromosoma·Bungo Akiyoshi, Sue Biggins
Mar 21, 2013·Chromosoma·Hironori Funabiki, David J Wynne
Feb 12, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·John Tooley, P Todd Stukenberg
Mar 23, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Han Zhang, R Kelly Dawe
Jul 18, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·António J Pereira, Helder Maiato
Sep 10, 2010·Journal of Biosciences·Amit Bardhan
Mar 14, 2013·Trends in Cell Biology·Stefan Westermann, Alexander Schleiffer
Apr 20, 2013·Trends in Biochemical Sciences·Luying JiaHongtao Yu
Mar 23, 2013·Cell Death and Differentiation·S HagemannH A Baba
Oct 15, 2010·Nature·Gregory M AlushinEva Nogales
Feb 2, 2010·Nature Cell Biology·Keith T Jones
Oct 5, 2010·Nature Cell Biology·Alexey Khodjakov, Jonathon Pines
Mar 17, 2010·Nature Cell Biology·Ana C AmaroPatrick Meraldi
May 9, 2012·Nature Cell Biology·Alexander SchleifferStefan Westermann
May 19, 2011·Nature Communications·Adrian T SaurinGeert J P L Kops
Apr 21, 2009·Nature Reviews. Cancer·John L Nitiss
Nov 26, 2010·Nature Reviews. Cancer·Susanne M A LensRené H Medema
Jan 14, 2010·Nature Reviews. Molecular Cell Biology·Claire E WalczakAlexey Khodjakov
Jun 3, 2011·Nature Reviews. Molecular Cell Biology·Fabienne Lampert, Stefan Westermann
Jul 14, 2011·Nature Reviews. Molecular Cell Biology·Claudia Wurzenberger, Daniel W Gerlich
May 17, 2012·Nature Reviews. Molecular Cell Biology·Yoshinori Watanabe
Nov 24, 2012·Nature Reviews. Molecular Cell Biology·Mar CarmenaWilliam C Earnshaw
Dec 22, 2012·Nature Reviews. Molecular Cell Biology·Emily A Foley, Tarun M Kapoor
Apr 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Takeshi ItabashiShin'ichi Ishiwata
Dec 18, 2013·The Journal of Cell Biology·Gina V CaldasJennifer G DeLuca
Jul 17, 2009·Molecular Biology of the Cell·Tessie M NgSue Biggins
Apr 27, 2012·Molecular Biology of the Cell·Emily A HoodDuane A Compton
May 4, 2012·Molecular Biology of the Cell·Friederike AlthoffChristian F Lehner
Dec 15, 2010·Journal of Molecular Cell Biology·Youjun ChuXuebiao Yao
Dec 29, 2012·Open Biology·Hauke Drechsler, Andrew D McAinsh
Mar 22, 2013·Open Biology·Helfrid HocheggerJose B Pereira-Leal
Nov 16, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Andrea Musacchio
Nov 16, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jonathon Pines, Iain Hagan
Nov 16, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Frank UhlmannSandra López-Avilés
Dec 17, 2009·Genes & Development·Vincent Vanoosthuyse, Kevin G Hardwick
Oct 5, 2010·Genes & Development·Yuji TannoYoshinori Watanabe
Oct 12, 2010·Science·Yuya YamagishiYoshinori Watanabe

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