Sensing intracellular calcium ions using a manganese-based MRI contrast agent

Nature Communications
Ali BarandovAlan Jasanoff

Abstract

Calcium ions are essential to signal transduction in virtually all cells, where they coordinate processes ranging from embryogenesis to neural function. Although optical probes for intracellular calcium imaging have been available for decades, the development of probes for noninvasive detection of intracellular calcium signaling in deep tissue and intact organisms remains a challenge. To address this problem, we synthesized a manganese-based paramagnetic contrast agent, ManICS1-AM, designed to permeate cells, undergo esterase cleavage, and allow intracellular calcium levels to be monitored by magnetic resonance imaging (MRI). Cells loaded with ManICS1-AM show changes in MRI contrast when stimulated with pharmacological agents or optogenetic tools; responses directly parallel the signals obtained using fluorescent calcium indicators. Introduction of ManICS1-AM into rodent brains furthermore permits MRI-based measurement of neural activation in optically inaccessible brain regions. These results thus validate ManICS1-AM as a calcium sensor compatible with the extensive penetration depth and field of view afforded by MRI.

References

Jan 1, 1979·Annual Review of Physiology·G G Somjen
Sep 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tatjana AtanasijevicAlan Jasanoff
Jul 8, 2008·Chembiochem : a European Journal of Chemical Biology·Goran AngelovskiNikos K Logothetis
Oct 7, 2008·Nature Methods·Benjamin A FlusbergMark J Schnitzer
Jan 30, 2009·Medical Physics·Lihong V Wang
Mar 7, 2009·Accounts of Chemical Research·Luis M De Leon-RodriguezA Dean Sherry
Dec 22, 2009·Chemical Society Reviews·Emily L Que, Christopher J Chang
Mar 6, 2010·Journal of the American Chemical Society·Duangkhae SrikunChristopher J Chang
Jul 9, 2010·Chemistry & Biology·Taekwan LeeAlan Jasanoff
Mar 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lin TianLuke D Lavis
Apr 5, 2012·Chemical Communications : Chem Comm·Sayoko YamamotoKoichi Kato
Oct 23, 2012·Neuron·Qian ChenGuoping Feng
Dec 18, 2013·Chemical Reviews·Marie C HeffernThomas J Meade
Aug 19, 2015·Nature Communications·Tomohiro IshiiTakao Nakata
Apr 15, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Benjamin B BartelleAlan Jasanoff
Apr 19, 2016·Journal of the American Chemical Society·Ali BarandovAlan Jasanoff
Sep 26, 2017·Journal of the American Chemical Society·Sheryl RobertsGil G Westmeyer
Apr 13, 2018·Current Opinion in Neurobiology·Souparno GhoshAlan Jasanoff
May 2, 2018·Nature Nanotechnology·Satoshi OkadaAlan Jasanoff
Dec 2, 2016·Light, Science & Applications·X Luís Deán-BenDaniel Razansky

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Citations

Jan 14, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Francesca GarelloEnzo Terreno
Mar 5, 2020·Chemical Communications : Chem Comm·Giuseppe GambinoGoran Angelovski
Apr 1, 2020·Chemical Society Reviews·Adam C SedgwickTony D James
Aug 9, 2020·Analytical and Bioanalytical Chemistry·Hang SuXiao Yong Tong
Aug 26, 2020·Chemical Communications : Chem Comm·Markus F K TrautnitzMichael Seitz
Sep 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tanja SavićGoran Angelovski
Jan 23, 2020·ACS Chemical Biology·Casey J AdamsThomas J Meade
Mar 12, 2021·Current Opinion in Chemical Biology·Célia S Bonnet, Éva Tóth
May 22, 2020·ACS Sensors·Ali BarandovAlan Jasanoff
Jun 5, 2021·Chemical Physics Reviews·Austin D C MillerArnab Mukherjee
Jul 24, 2021·Journal of the American Chemical Society·Nishanth D TirukotiAmnon Bar-Shir
Oct 9, 2019·Journal of the American Chemical Society·Hao Li, Thomas J Meade
Aug 24, 2021·ACS Sensors·Harun F OzbakirArnab Mukherjee
Oct 17, 2021·Proceedings of the National Academy of Sciences of the United States of America·He WeiAlan Jasanoff

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Methods Mentioned

BETA
imaging techniques
fluorescence spectroscopy

Software Mentioned

MATLAB scripts
Arduino
ImageJ
ManICS1
MRI Analysis Calculator Plugin for ImageJ
AM

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