Sensing minute changes in biological cell monolayers with THz differential time-domain spectroscopy

Biosensors & Bioelectronics
Hai-Bo LiuX-C Zhang

Abstract

We used terahertz differential time-domain spectroscopy (THz-DTDS) to measure minute changes of bovine lung microvessel endothelial cells (BLMVEC) in response to vascular endothelial growth factor (VEGF). These changes were reflected by alterations in THz wave attenuations and THz dielectric properties of the treated cells. The VEGF-induced THz attenuations of cell monolayers correlated well with changes in transendothelial resistance, as measured using electric cell-substrate impedance sensing (ECIS). However, the morphological differences that gave rise to these changes were not observed with standard optical phase contrast microscopy. We conclude that THz-DTDS is a highly sensitive, non-invasive, powerful new tool to measure minute changes in the morphology of live, cultured cell monolayers. This method enables spectroscopic investigations of cells in the THz band, providing information unavailable through other conventional methods such as optical phase contrast microscopy and ECIS.

References

Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·I Giaever, C R Keese
Dec 9, 1993·Nature·I Giaever, C R Keese
Apr 3, 1996·Biochimica Et Biophysica Acta·R LisinY Feldman
Nov 28, 2002·Physics in Medicine and Biology·Samuel P MickanX C Zhang
Nov 28, 2002·Physics in Medicine and Biology·Andrea MarkelzRobert Birge
Nov 28, 2002·Physics in Medicine and Biology·B M FischerP Uhd Jepsen
Nov 28, 2002·Physics in Medicine and Biology·Ruth M WoodwardMichael Pepper
Oct 21, 2004·Biosensors & Bioelectronics·Abdellah MenikhX-C Zhang

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Citations

Aug 22, 2007·The Analyst·Fareid Asphahani, Miqin Zhang
May 26, 2009·Biotechnology Advances·Jixian GongXueming Zhao
Nov 5, 2008·Journal of Biomedical Materials Research. Part a·Dario ColettiMario Barteri
May 2, 2016·Applied Microbiology and Biotechnology·Xiang YangWeiling Fu
May 22, 2016·Trends in Biotechnology·Xiang YangYang Luo
Jun 10, 2010·Journal of Biophotonics·Changlei WangAleksei M Zheltikov
Aug 20, 2010·Optics Express·Maik SchellerMartin Koch
Feb 12, 2014·Optics Express·Withawat WithayachumnankulWeili Zhang
Jun 15, 2013·Electrophoresis·Yung-Sheng LinHaw-Ming Huang
Mar 11, 2020·Cell Proliferation·Zaoxia LiHuabin Wang
Jun 22, 2019·Proceedings of the National Academy of Sciences of the United States of America·Seung-Min LimJeong-Yun Sun
Jun 2, 2011·Journal of Neural Engineering·R W F WiertzW L C Rutten
Apr 7, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yuqi CaoShu Zheng

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