Sensing of biomolecular interactions using fluorescence complementing systems in living cells

Biosensors & Bioelectronics
Xian-En ZhangDianbing Wang

Abstract

Sensing biomolecule interactions in living cells allows for a deeper understanding of the mechanisms governing biological processes, and has increasing significance for improvements in clinical diagnosis. It is now possible by using molecular biosensors. One method involving molecular biosensors is called molecular fluorescence complementation, usually referred to as BiFC (bimolecular fragment/fluorescence complementary/complementation) or TriFC (trimolecular fragment complementary/complementation). This complementation method is based on the principle that two non-fluorescent fragments of a fluorescent protein are brought into sufficient lyclose proximity, upon which they are reconstructed so that fluorescence is re-established. This process relies on the interaction between the two fusion partners, which normally are proteins. This method is simple, noninvasive, sensitive, and does not require specialized tools, hence being available to most standard laboratories. Here, we selectively describe three relevant examples, although many other molecular interactions have been shown to work with this method. Recent developments of this method include multicolor BiFC, which allows for simultaneous detection of multi-biomolecule inter...Continue Reading

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Citations

Jan 9, 2019·SLAS Discovery·Vito Giuseppe D'AgostinoValentina Adami
Dec 19, 2015·Molecular Systems Biology·Jamie SniderIgor Stagljar
Aug 9, 2017·Expert Review of Proteomics·Alla GagarinovaMohan Babu
Jun 20, 2020·Science China. Life Sciences·Xian-En Zhang
Jun 20, 2019·Biochemistry. Biokhimii︠a︡·M M KarasevV V Verkhusha
Sep 30, 2021·Molecular Microbiology·Jessica KimmelTobias Spielmann

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