Sensing of Escherichia coli and LPS by mammary epithelial cells is modulated by O-antigen chain and CD14

PloS One
Mégane VédrineP Germon

Abstract

Escherichia coli is one of the major pathogens causing mastitis in dairy cattle. Yet, the factors which mediate the ability for E. coli to develop in the bovine mammary gland remain poorly elucidated. In a mouse model, infections induced by the reference mastitis E. coli P4 showed a strong colonisation of the mammary gland, while this strain had a low stimulating power on cells of the PS bovine mammary epithelial cell line. In order to understand if such a reduced response contributes to the severity of infection, a library of random mutants of P4 strain was screened to identify mutants inducing stronger response of PS cells. Among hyper-stimulating mutants, six were altered in genes involved in biosynthesis of lipopolysaccharide (LPS) and had lost their O-polysaccharide region, suggesting that the presence of O-antigen impairs the response of PS cells to LPS. Using purified smooth (S) and rough (R) fractions of LPS, we showed that the R-LPS fraction induced a stronger response from PS cells than the smooth LPS fraction. Biological activity of the S-LPS fraction could be restored by the addition of recombinant bovine CD14 (rbCD14), indicating a crucial role of CD14 in the recognition of S-LPS by Mammary Epithelial Cells (MEC). ...Continue Reading

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Citations

May 20, 2020·The Journal of Biological Chemistry·Chris WhitfieldSteven D Kelly
Jan 21, 2021·Animals : an Open Access Journal From MDPI·Efstathios Sarmikasoglou, Antonio P Faciola
Oct 17, 2020·Veterinary Research·Pascal RainardGilles Foucras

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BETA
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enzyme-linked immunosorbent assay
electrophoresis
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Reverse
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