Sensing of silver nanoparticles on/in endothelial cells using atomic force spectroscopy

Journal of Molecular Recognition : JMR
Agnieszka KolodziejczykBogdan Walkowiak

Abstract

Endothelial cells, due to their location, are interesting objects for atomic force spectroscopy study. They constitute a barrier between blood and vessel tissues located deeper, and therefore they are the first line of contact with various substances present in blood, eg, drugs or nanoparticles. This work intends to verify whether the mechanical response of immortalized human umbilical vein endothelial cells (EA.hy926), when exposed to silver nanoparticles, as measured using force spectroscopy, could be effectively used as a bio-indicator of the physiological state of the cells. Silver nanoparticles were characterized with transmission electron microscopy and dynamic light scattering techniques. Tetrazolium salt reduction test was used to determine cell viability after treatment with silver nanoparticles. An elasticity of native cells was examined in the Hanks' buffer whereas fixed cells were softly fixed with formaldehyde. Additional aspect of the work is the comparative force spectroscopy utilizing AFM probes of ball-shape and conical geometries, in order to understand what changes in cell elasticity, caused by SNPs, were detectable with each probe. As a supplement to elasticity studies, cell morphology observation by atomic ...Continue Reading

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Citations

May 2, 2020·Journal of Molecular Recognition : JMR·Agnieszka M KolodziejczykBogdan Walkowiak
Dec 20, 2018·Journal of Molecular Recognition : JMR·Jean-Luc PellequerFelix Rico
Mar 27, 2021·Micron : the International Research and Review Journal for Microscopy·Agnieszka Maria KolodziejczykBogdan Walkowiak
Dec 26, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Paulina SokołowskaBogdan Walkowiak

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