Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes

Frontiers in Chemistry
Maria WeberTony D James

Abstract

The elucidation of biological processes involving reactive oxygen species (ROS) facilitates a better understanding of the underlying progression of non-communicable diseases. Fluorescent probes are a powerful tool to study various ROS and have the potential to become essential diagnostic tools. We have developed a series of coumarin fluorescent probes for the selective and sensitive detection of peroxynitrite (ONOO-), a key ROS. Coumarin based probes exhibit good photostability, large Stokes shift and high quantum yields. The three ratiometric probes all contain a boronate ester motif for the detection of ONOO- and a distinctive organelle targeting group. The study of ONOO- generation in a particular organelle will allow more precise disease profiling. Hence, targeting groups for the mitochondria, lysosome and endoplasmic reticulum were introduced into a coumarin scaffold. The three ratiometric probes displayed sensitive and selective detection of ONOO- over other ROS species. All three coumarin probes were evaluated in murine RAW264.7 macrophages for detection of basal and stimulated ONOO- formation.

References

Dec 3, 2003·Chemical Communications : Chem Comm·Lee-Chiang Lo, Chi-Yuan Chu
Feb 14, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nobuaki SohToshihiko Imato
Nov 1, 2011·Chemical Communications : Chem Comm·Lin YuanYueting Yang
Aug 6, 2015·Chemical Biology & Drug Design·Ehtesham JameelNasimul Hoda
Oct 19, 2016·Accounts of Chemical Research·Hao ZhuXiaojun Peng
Jan 26, 2017·Frontiers in Cellular Neuroscience·Radhika RastogiYuchuan Ding
Oct 12, 2017·Chemical Society Reviews·Di WuTony D James
Dec 30, 2017·International Journal of Molecular Sciences·Daisuke HirayamaHiroshi Nakase
Jul 13, 2018·Chemical Communications : Chem Comm·Maria L OdyniecTony D James
Aug 18, 2018·Chemical Communications : Chem Comm·Luling WuTony D James
Sep 13, 2018·Cancer Research·Ming YangClaire E Lewis
Mar 13, 2019·Journal of Molecular Cell Biology·Yufang WangZhaoyu Lin
Jul 10, 2019·International Journal of Molecular Sciences·Nathan KelleyYuan He
Aug 14, 2019·Autoimmunity Reviews·Paola Di BenedettoRoberto Giacomelli
Jan 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bingpeng GuoXiaoling Zhang

❮ Previous
Next ❯

Citations

Oct 27, 2020·Frontiers in Chemistry·Adam SikoraBalaraman Kalyanaraman

❮ Previous
Next ❯

Methods Mentioned

BETA
PMA
confocal microscopy

Software Mentioned

Elmer UVWinlab
Perkin
Image J
BMG Labtech MARS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.